Quantitative Trait Loci for Clubroot Resistance in Brassica oleracea

  • R. E. Voorrips
  • M. C. Jongerius
  • H. J. Kanne
Part of the Biotechnology in Agriculture and Forestry book series (AGRICULTURE, volume 52)


Clubroot, caused by the fungus Plasmodiophora brassicae Wor., is one of the most damaging diseases of Brassica oleracea crops, including cauliflower, broccoli, Brussels sprouts, cabbage, and other cruciferous crops worldwide. The pathogen causes swelling of parts of the roots and sometimes of the stem base, giving rise to the characteristic “clubs” for which the disease is named (Fig. 1). These clubs inhibit nutrient and water transport, stunt the growth of the plant, and increase the susceptibility to wilting. Later on, the clubbed roots decay, weakening the support of the plant.


Quantitative Trait Locus Linkage Group Brassica Oleracea Microspore Culture Clubroot Resistance 
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  1. Bernatzky R, Tanksley SD (1986) Toward a saturated linkage map in tomato based on isozymes and random cDNA sequences. Genetics 112:887–898PubMedGoogle Scholar
  2. Buczacki ST, Toxopeus H, Mattusch P, Johnston TD, Dixon GR, Hobolth LA (1975) Study of physiologic specialization in Plasmodiophora brassicae: proposals for attempted rationalization through an international approach. Trans Br Mycol Soc 65:295–303CrossRefGoogle Scholar
  3. Chiang MS, Crête R (1970) Inheritance of clubroot resistance in cabbage (Brassica oleracea L. var. capitata L.). Can J Genet Cytol 12:253–256Google Scholar
  4. Chiang MS, Crête R (1976) Diallele analysis of the inheritance of resistance to race 6 of Plasmodiophora brassicae in cabbage. Can J Plant Sci 56:865–868CrossRefGoogle Scholar
  5. Cloutier S, Cappadocia M, Landry BS (1995) Study of microspore-culture responsiveness in oilseed rape (Brassica napus L.) by comparative mapping of a F<Subscript>2</Subscript> population and two microspore-derived populations. Theor Appl Genet 91:841–847CrossRefGoogle Scholar
  6. Crête R (1981) Worldwide importance of clubroot. Clubroot Newsl 11:6–7Google Scholar
  7. Duijs JG, Voorrips RE, Visser DL, Custers JBM (1992) Microspore culture is successful in most crop types of Brassica oleracea L. Euphytica 60:45–55Google Scholar
  8. Feinberg AP, Vogelstein B (1983) A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal Biochem 132:6–13PubMedCrossRefGoogle Scholar
  9. Ferreira ME, Williams PH, Osborn TC (1995) RFLP mapping of Brassica napus using doubled haploid lines. Theor Appl Genet 89:615–621Google Scholar
  10. Figdore SS, Ferreira ME, Slocum MK, Williams PH (1993) Association of RFLP markers with trait loci affecting clubroot resistance and morphological characters in Brassica oleracea L. Euphytica 69:33–44CrossRefGoogle Scholar
  11. Grand Clément C, Thomas G (1996) Detection and analysis of QTLs based on RAPD markers for polygenic resistance to Plasmodiophora brassicae Woron in Brassica oleracea L. Theor Appl Genet 93:86–90CrossRefGoogle Scholar
  12. Harada JJ, Baden CS, Cornai L (1988) Spatially regulated genes expressed during seed germination and postgerminative development are activated during embryogeny. Mol Gen Genet 212:466–473CrossRefGoogle Scholar
  13. Hu J, Sadowski J, Osborn TC, Landry BS, Quiros CF (1998) Linkage group alignment from four independent Brassica oleracea RFLP maps. Genome 41:226–235Google Scholar
  14. Jansen RC (1994) Controlling the type I and type II errors in mapping quantitative trait loci. Genetics 138:871–881PubMedGoogle Scholar
  15. Jansen RC, Stam P (1994) High resolution of quantitative traits into multiple loci via interval mapping. Genetics 136:1447–1455PubMedGoogle Scholar
  16. Keller WA, Armstrong KC (1981) Production of anther-derived dihaploid plants in autotetraploid marrowstem kale (Brassica oleracea var. acephala). Can J Genet Cytol 23:259–265Google Scholar
  17. Kennard WC, Slocum MK, Figdore SS, Osborn TC (1994) Genetic analysis of morphological variation in Brassica oleracea using molecular markers. Theor Appl Genet 87:721–732CrossRefGoogle Scholar
  18. Kianian SF, Quiros CF (1992) Generation of a Brassica oleracea composite RFLP map—linkage arrangements among various populations and evolutionary implications. Theor Appl Genet 84:544–554CrossRefGoogle Scholar
  19. Kosambi DD (1944) The estimation of map distances from recombination values. Ann Eugenics 12:172–175Google Scholar
  20. Landry BS, Hubert N, Crête R, Chiang M, Lincoln SE, Etoh T (1992) A genetic map for Brassica oleracea based on RFLP markers detected with expressed DNA sequences and mapping of resistance genes to race 2 of Plasmodiophora brassicae (Woronin). Genome 35:409–420Google Scholar
  21. Lichter R (1982) Induction of haploid plants from isolated pollen of Brassica napus. Z Pflanzen-physiol 105:427–434Google Scholar
  22. Mattusch P (1977) Epidemiology of clubroot of crucifers caused by Plasmodiophora brassicae. In: Buczacki ST, Williams PH (eds) Woronin +100 International Conference on Clubroot. University of Wisconsin, Madison, WI, pp 24–28aGoogle Scholar
  23. Moriguchi K, Kimizuka-Takagi C, Ishii K, Nomura K (1999) A genetic map based on RAPD, RFLP, isozyme, morphological markers and QTL analysis for clubroot resistance in Brassica oleracea. Theor Appl Genet 93:86–90Google Scholar
  24. Murakami H, Tsushima S, Shishido Y (2000) Soil suppressiveness to clubroot disease of Chinese cabbage caused by Plasmodiophora brassicae. Soil Biol Chem 32:11–12CrossRefGoogle Scholar
  25. Orton TJ, Browers MA (1985) Segregation of genetic markers among plants regenerated from cultured anthers of broccoli (Brassica oleracea var. italica). Theor Appl Genet 69:637–643CrossRefGoogle Scholar
  26. Rick CM (1983) Tomato (Lycopersicon). In:Tanksley SD, Orton TJ (eds) Isozymes in plant genetics breeding, part B. Elsevier, Amsterdam, pp 147–165Google Scholar
  27. Sebastian RL, Howell EC, King GJ, Marshall DF, Kearsey MJ (2000) An integrated AFLP and RFLP Brassica oleracea linkage map from two morphologically distinct doubled-haploid mapping populations. Theor Appl Genet 100:75–81CrossRefGoogle Scholar
  28. Slocum MK, Figdore SS, Kennard WC, Suzuki JY, Osborn TC (1990) Linkage arrangement of restriction fragment length polymorphism loci in Brassica oleracea. Theor Appl Genet 80:57–64CrossRefGoogle Scholar
  29. Stam P (1993) Construction of integrated genetic linkage maps by means of a new computer package: JoinMap. Plant J 3:739–744CrossRefGoogle Scholar
  30. Stam P, Van Ooijen JW (1995) Join Map(TM) version 2.0: software for the calculation of genetic linkage maps. CPRO-DLO, Wageningen, NetherlandsGoogle Scholar
  31. Tanhuanpää PK, Vilkki JP, Vilkki HJ (1994) Segregation and linkage analysis of DNA markers in microspore derived and F<Subscript>2</Subscript> populations of oilseed rape (Brassica napus L). Euphytica 74:59–65CrossRefGoogle Scholar
  32. Van der Beek JG, Verkerk R, Zabel P, Lindhout P (1992) Mapping strategy for resistance genes in tomato based on RFLPs between cultivars: Cf9 (resistance to Cladosporium fulvum) on chromosome 1. Theor Appl Genet 84:106–112CrossRefGoogle Scholar
  33. Van der Beek JG, Pet G, Lindhout P (1994) Resistance to powdery mildew (Oidium lycopersicum) in Lycopersicon hirsutum is controlled by an incompletely dominant gene Ol-1 on chromosome 6. Theor Appl Genet 89:467–473CrossRefGoogle Scholar
  34. Van Ooijen JW (1992) Accuracy of mapping quantitative trait loci in autogamous species. Theor Appl Genet 84:803–811CrossRefGoogle Scholar
  35. Van Ooijen JW, Maliepaard C (1995) MapQTL version 3.0: software for the calculation of QTL positions on genetic maps. CPRO-DLO, Wageningen, NetherlandsGoogle Scholar
  36. Voorrips RE (1995) Plasmodiophora brassicae: aspects of pathogenesis and resistance in Brassica oleracea. Euphytica 83:139–146CrossRefGoogle Scholar
  37. Voorrips RE (2002) MapChart: software for the graphical presentation of linkage maps and QTLs. J Hered 93:77–78PubMedCrossRefGoogle Scholar
  38. Voorrips RE, Kanne HJ (1997a) Genetic analysis of resistance to clubroot (Plasmodiophora brassicae) in Brassica oleracea. 1. Analysis of symptom grades. Euphytica 93:31–39Google Scholar
  39. Voorrips RE, Kanne HJ (1997b) Genetic analysis of resistance to clubroot (Plasmodiophora brassicae) in Brassica oleracea. 2. Quantitative analysis of root symptom measurements. Euphytica 93:41–48CrossRefGoogle Scholar
  40. Voorrips RE, Visser DL (1993) Examination of resistance to clubroot in accessions of Brassica oleracea using a glasshouse seedling test. Neth J Plant Pathol 99:269–276CrossRefGoogle Scholar
  41. Vos P, Hogers R, Bleeker M, Reijans M, Van de Lee T, Homes M, Frijters A, Pot J, Peleman J, Kuiper M, Zabeau M (1995) AFLP: a new technique for DNA fingerprinting. Nucleic Acids Res 23:4407–4414PubMedCrossRefGoogle Scholar
  42. Williams PH (1966) A system for the determination of races of Plasmo diophora brassicae that infect cabbage and rutabaga. Phytopathology 56:624–626Google Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2003

Authors and Affiliations

  • R. E. Voorrips
    • 1
  • M. C. Jongerius
    • 1
  • H. J. Kanne
    • 1
  1. 1.Plant Research International B.V.WageningenThe Netherlands

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