Application of Molecular Markers in Brassica Coenospecies: Comparative Mapping and Tagging

  • M. Lakshmikumaran
  • S. Das
  • P. S. Srivastava
Part of the Biotechnology in Agriculture and Forestry book series (AGRICULTURE, volume 52)


The great bulk of genetic variation at the nucleotide level may not be visible at the phenotypic level. It is this variation that is exploited to generate molecular markers. The study of molecular genetics and plant breeding has been aided variously and to an unprecedented degree and accuracy with the developments in the field of molecular markers. Techniques such as restriction fragment length polymorphism (RFLP), randomly amplified polymorphic DNA (RAPD), amplified fragment length polymorphism (AFLP), simple sequence repeats (SSR), expressed sequence tags (EST), cleaved amplified polymorphic sequence (CAPS), sequence characterized amplified region (SCAR) and single nucleotide polymorphisms (SNP) have permitted rapid and precise analysis of germplasm, trait mapping, and marker-assisted breeding and selection. These markers have also been used to further our insight into genome evolution, and to study gene order in terms of conservation (synteny) and rearrangement across taxa. Molecular or DNA-based markers offer many advantages over conventional phenotypic markers. They are heritable, easy to score, free from developmental and environmental influences, detectable in all tissues, insensitive to epistatic or pleiotropic interactions, and they provide a choice of codominant (simultaneous detection of various alleles) or dominant (no allelic information conveyed) markers.


Amplify Fragment Length Polymorphism Erucic Acid Amplify Fragment Length Polymorphism Marker Cleave Amplify Polymorphic Sequence Brassica Species 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Copyright information

© Springer-Verlag Berlin Heidelberg 2003

Authors and Affiliations

  • M. Lakshmikumaran
    • 1
  • S. Das
    • 2
  • P. S. Srivastava
    • 2
  1. 1.Bioresources and Biotechnology DivisionTERI, Habitat PlaceNew DelhiIndia
  2. 2.Centre for Biotechnology, Faculty of ScienceHamdard UniversityHamdard Nagar, New DelhiIndia

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