Multipass Cuvettes for Luminescence Spectroscopy

Abstract

Fluorescence spectroscopy is one of the most sensitive and widely used experimental techniques in physics, chemistry and biology. One of the ways of enhancing sensitivity in fluorescence spectroscopy is increasing the optical path of exciting light in the fluorescent sample. Exciting light passes through the sample two or three times due to reflections from the mirrors placed near the cell filled with the fluorescent solution under study [152–154]. The gain in sensitivity does not exceed a factor of 2 or 3 and also light losses at the interfaces. The mirror-air interface induces an artifact polarization of light. Moreover, due to the reflections at the interfaces the exciting light can get in the path of the emission light, contaminating fluorescence signal.