Pyrene Monomers and Excimers in Membranes
The aromatic hydrocarbon pyrene is widely applied as a luminescence probe sensitive to the physico-chemical state of biological and model lipid membranes [1921, 60, 369–372, 375, 382, 421–423]. Very long lifetime allows pyrene to be high sensitive to viscosity, temperature, etc. The pyrene “boom” was initiated by the simultaneously published reports by Vanderkooi & Callis  and Galla & Sackmann , where the ability of pyrene to form excimers was used as a basis for a new approach to the measurement of membrane viscosity. This extrapolation was borrowed from well known studies of the dependence of the rate of pyrene excimerization in organic solvents on their viscosity, which affects molecular diffusion (reviews [138, 151, 52–55, 57, 162]).
KeywordsSucrose Phenol Albumin Hydrocarbon Tryptophan
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