Abstract
In current transformation systems, a selectable marker gene is co-delivered with the gene of interest to identify and separate rare transgenic cells from non-transgenic cells. Since, during transformation, only a few plant cells accept the integration of foreign DNA, most of the cells remain non-transgenic. Usually, conditional dominant genes, which have no influence on the growth or morphology of plants, are used as selectable markers because they remain in the transgenic plants after transformation. The corresponding selective agents, which inhibit the growth of non-transgenic cells, are applied to the culture medium to identify transgenic plants. However, these selection systems have three potential pitfalls. (1) The negative effects of selective agents decrease the ability of transgenic cells to proliferate and differentiate into transgenic plants. (2) The presence of marker genes in transgenic plants precludes the use of the same marker gene for gene stacking through re-transformation. (3) The recent public concerns regarding the release of antibiotic-resistance genes limit their use for the commercialization of transgenic crops.
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Ebinuma, H., Sugita, K., Matsunaga, E., Endo, S., Yamada, K. (2002). GST-MAT Vector for the Efficient and Practical Removal of Marker Genes from Transgenic Plants. In: Jackson, J.F., Linskens, H.F. (eds) Testing for Genetic Manipulation in Plants. Molecular Methods of Plant Analysis, vol 22. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-04904-4_7
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DOI: https://doi.org/10.1007/978-3-662-04904-4_7
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