RNA Gel Blot Analysis to Determine Gene Expression of Floral Scents
To attract pollinators to flowers, flowering plants use diverse biosynthetic pathways to produce a broad spectrum of low molecular weight (100- to 200-Da) floral volatile compounds (>700 described structures exist in 60 families of plants) (Knudsen et al. 1993; Dobson 1994). In most plant species floral volatiles are emitted primarily by the petals, although other parts of the flower may also contribute to the total scent output. Often, a specific floral component is emitted from several parts of the flower, although not necessarily at the same amount or rate. However, there are also examples of emission of specific compounds from only a subset of the floral organs.
KeywordsFloral Scent Methyl Benzoate Floral Tissue Determine Gene Expression Floral Volatile
Unable to display preview. Download preview PDF.
- Dobson HEM (1994) Floral volatiles in insect biology. In: Bernays E (ed) Insect-plant interactions, vol 5. CRC Press, Boca Raton, pp 47–81Google Scholar
- Dudareva N, Piechulla B, Pichersky E (1999) Biogenesis of floral scent. Hortic Rev 24:31–54Google Scholar
- McCaskill D, Croteau R (1995) Isoprenoid synthesis in peppermint (Mentha X piperita): development of a model system for measuring flux of intermediates through the mevalonic acid pathway in plants. Biochem Soc Trans 23:290SGoogle Scholar
- Murfitt LM, Kolosova N, Mann CJ, Dudareva N (2000) Purification and characterization of S-adenosyl-L-methionine:benzoic acid carboxyl methyltransferase, the enzyme responsible for biosynthesis of the volatile ester methyl benzoate in flowers of Antirrhinum majus. Arch Biochem Biophys 382:145–151PubMedCrossRefGoogle Scholar
- Wang J, Dudareva N, Bhakta S, Raguso RA, Pichersky E (1997) Floral scent production in Clarkia breweri (Onagraceae). II. Localization and developmental modulation of the enzyme S-adenosyl-L-methionine:(iso)eugenol O-methyltransferase and phenylpropanoid emission. Plant Physiol 114:213–221PubMedCrossRefGoogle Scholar