Abstract
Complementary DNAs encoding four G-protein subunits (Gαi, Gαs, Gαq, and Gβ), a phospholipase C-β (lobPLCβ), and a G-protein coupled receptor kinase (lobGRK2) were isolated from lobster olfactory organ cDNA libraries. All five proteins were detected in the olfactory dendrites. Functional assays demonstrated that Gαq, Gβ, lobPLCβ, and lobGRK2 were sensitive to stimulation with odorants. These results imply that Gαq, Gβ, and lobPLCβ are directly involved in excitatory olfactory transduction, and that lobGRK2 participates in desensitization. Similar evidence indicates that these same gene products are also involved in neurotransmission in the brain. The cDNA clones are expressed in the brain, and the proteins they encode are most abundant in brain neuropil. In brain homogenates, Gαq, Gβ, lobPLCβ, and lobGRK2 were sensitive to stimulation with neurotransmitters. In addition, we found that several neurotransmitters stimulated labeling of Gαi by a GTP photoaffmity analog. These results indicate that the lobster brain is richly endowed with neuromodulator/ G-protein pathways.
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© 2002 Springer-Verlag Berlin Heidelberg
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McClintock, T.S., Xu, F. (2002). Molecular Physiology of G-Proteins in Olfactory Transduction and CNS Neurotransmission in the Lobster. In: Wiese, K. (eds) The Crustacean Nervous System. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-04843-6_27
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DOI: https://doi.org/10.1007/978-3-662-04843-6_27
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