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Enzyme Kinetics: Stopped-Flow Under Extreme Conditions

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Biological Systems Under Extreme Conditions

Part of the book series: Biological and Medical Physics Series ((BIOMEDICAL))

Abstract

Temperature and pressure (extreme conditions), as perturbing variables, are two powerful tools to investigate the thermodynamic parameters of chemical or biochemical reactions and to study the mechanism of enzyme-catalyzed reactions, via the determination of rate constants and the use of the transition-state theory. However, one difficulty in measuring kinetics using spectroscopic detection is the relative long dead-time of the low-temperature and the high-pressure techniques. Different devices have been proposed to eliminate sources of errors in time, temperature and pressure. These techniques include in situ initiation of the reaction after temperature and pressure equilibrium. Using different examples of reactions, the interdependence of these two major variables, namely temperature and pressure, is presented in this chapter, in which the role of the reaction media is considered as a third variable.

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Author information

Authors and Affiliations

  1. INSERM, Unité 128, IFR 24, CNRS, 1919, route de Mende, 34293, Montpellier Cedex 5, France

    Claude Balny

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  1. Claude Balny
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Editors and Affiliations

  1. Department of Applied Chemistry, Faculty of Science and Engineering, Ritsumeikan University, 1-1-1, Nojihigshi, Kusatsu, 525-8577, Shiga, Japan

    Yoshihiro Taniguchi

  2. Center for Polymer Studies and Department of Physics, Boston University, 02215, Boston, MA, USA

    Harry E. Stanley

  3. Institute of Pharmaceutical Technology and Biopharmacy, University of Heidelberg, Im Neuenheimer Feld 346, 69120, Heidelberg, Germany

    Horst Ludwig

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© 2002 Springer-Verlag Berlin Heidelberg

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Balny, C. (2002). Enzyme Kinetics: Stopped-Flow Under Extreme Conditions. In: Taniguchi, Y., Stanley, H.E., Ludwig, H. (eds) Biological Systems Under Extreme Conditions. Biological and Medical Physics Series. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-04802-3_8

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  • DOI: https://doi.org/10.1007/978-3-662-04802-3_8

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-08528-4

  • Online ISBN: 978-3-662-04802-3

  • eBook Packages: Springer Book Archive

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