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High-Pressure NMR Spectroscopy of Proteins

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Biological Systems Under Extreme Conditions

Part of the book series: Biological and Medical Physics Series ((BIOMEDICAL))

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Abstract

Advanced high-resolution NMR spectroscopy, including 2D NMR techniques, combined with the high-pressure capability represents a powerful new tool in studies of proteins. Selected results taken from recent studies illustrate the high information content and the range of problems that can be investigated. Design features and performance characteristics of high-sensitivity, high-resolution, variable-temperature NMR probes operating at 500 MHz and at pressures up to 900 MPa are described. The main portion of this chapter deals with an overview of several recent studies or studies in progress from our laboratory using 1D and 2D high-resolution, high-pressure NMR spectroscopy to investigate the pressure-induced reversible unfolding and cold denaturation of proteins. The following proteins were studied: ribonuclease A, lysozyme, apomyoglobin, arc repressor, and ubiquitin.

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Authors and Affiliations

  1. School of Chemical Sciences, University of Illinois at Urbana-Champaign, 600 South Mathews Avenue, Urbana, IL, 61801, USA

    Lance Ballard

  2. Beckman Institute for Advanced Science and Technology, University of Illinois at Urbana-Champaign, 405 North Mathews Avenue, Urbana, IL, 61801, USA

    Jiri Jonas

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  1. Lance Ballard
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Editors and Affiliations

  1. Department of Applied Chemistry, Faculty of Science and Engineering, Ritsumeikan University, 1-1-1, Nojihigshi, Kusatsu, 525-8577, Shiga, Japan

    Yoshihiro Taniguchi

  2. Center for Polymer Studies and Department of Physics, Boston University, 02215, Boston, MA, USA

    Harry E. Stanley

  3. Institute of Pharmaceutical Technology and Biopharmacy, University of Heidelberg, Im Neuenheimer Feld 346, 69120, Heidelberg, Germany

    Horst Ludwig

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Ballard, L., Jonas, J. (2002). High-Pressure NMR Spectroscopy of Proteins. In: Taniguchi, Y., Stanley, H.E., Ludwig, H. (eds) Biological Systems Under Extreme Conditions. Biological and Medical Physics Series. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-04802-3_4

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  • DOI: https://doi.org/10.1007/978-3-662-04802-3_4

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-08528-4

  • Online ISBN: 978-3-662-04802-3

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