Cryopreservation of Hordeum (Barley)

  • Jun-Hui Wang
  • Chun-Nong Huang
Part of the Biotechnology in Agriculture and Forestry book series (AGRICULTURE, volume 50)


Barley is the fourth most important cereal after wheat, rice and maize. Although its distribution is generally similar to wheat, barley can be grown in much drier and colder regions than wheat. It is distributed mainly over the middle latitudes of the earth especially of the northern hemisphere. Russia, Canada, United States, some European and Asian temperate countries are the world’s leading producers of this crop.


Embryogenic Callus Hordeum Vulgare Immature Embryo Antifreeze Protein Embryogenic Cell Suspension 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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  1. Antikainen M, Griffith M (1997) Antifreeze protein accumulation in freezing-tolerant cereals. Physiol Plant 99: 423–432CrossRefGoogle Scholar
  2. Bajaj YPS (1976) Gene preservation through freeze-storage of plant cell, tissue and organ culture. Acta Hortic 63: 75–84Google Scholar
  3. Bajaj YPS (1979) Technology and prospects of cryopreservation of germplasm. Euphytica 28: 267–285CrossRefGoogle Scholar
  4. Bajaj YPS (ed) (1995) Cryopreservation of plant germplasm I. Biotechnology in agriculture and forestry, vol 32. Springer, Berlin Heidelberg New York, 507 ppCrossRefGoogle Scholar
  5. Blakesley D, Pask N, Henshaw GG, Fay MF (1996) Biotechnology and the conservation of forest genetic resources: in vitro strategies and cryopreservation. Plant Growth Regul 20: 11–16CrossRefGoogle Scholar
  6. Christou P, Ford TL, Kofron M (1991) Production of transgenic rice (Oryza sativa L.) plants from agronomically important indica and japonica varieties via electric discharge particle acceleration of exogenous DNA into immature zygotic embryos. Bio/Technology 9: 957–962CrossRefGoogle Scholar
  7. Close TJ (1996) Dehydrins: emergence of a biochemical role of a family of plant dehydration proteins. Physiol Plant 97: 795–803CrossRefGoogle Scholar
  8. Close TJ (1997) Dehydrins: a commonalty in the response of plant to dehydration and low temperature. Physiol Plant 100: 291–296CrossRefGoogle Scholar
  9. Close TJ, Kortt AA, Chandler PM (1989) A cDNA-based comparison of dehydration-induced proteins (dehydrins) in barley and corn. Plant Mol Biol 13: 95–108PubMedCrossRefGoogle Scholar
  10. Ellis RH, Roberts EH (1980) Improved equations for the predictions of seed longevity. Ann Bot 45: 13–30Google Scholar
  11. Engelmann F (1991) In vitro conservation of tropical plant germplasm — a review. Euphytica 57: 227–243CrossRefGoogle Scholar
  12. Fretz A, Lörz H (1995) Cryopreservation of in vitro cultures of barley (Hordeum vulgare L. and H. murinum L.) and transgenic cells of wheat (Triticum aestivum L.). J Plant Physiol 146: 489–496CrossRefGoogle Scholar
  13. Fretz A, Jähne A, Lörz H (1992) Cryopreservation of embryogenic suspension cultures of barley (Hordeum vulgare L.). Bot Acta 105: 140–145Google Scholar
  14. Gao D, Ma D (1996) Germplasm resource of barley. In: Lu L (ed) Barley science in China. Chinese Agriculture Press, Beijing, pp 153–173Google Scholar
  15. Hahne G, Lörz H (1987) Cryopreservation of embryogenic callus cultures from barley (Hordeum vulgare L.). Plant Breed 99: 330–332CrossRefGoogle Scholar
  16. Huang C-N, Cornej NJ, Bush DS, Jones RL (1986) Estimating viability of plant protoplasts using double and single staining. Protoplasma 135: 80–87CrossRefGoogle Scholar
  17. Huang C-N, Yan Q-F, Wang J-H, Yu Z-Y, Du Y (1992) Studies on cryopreservation of barley (Hordeum vulgare L.) immature embryos. Bull Sci Technol 8: 209–212Google Scholar
  18. Huang C-N, Yan H, Yan Q, Zhu M, Yuan M, Xu A (1993) Establishment and characterization of embryogenic cell suspension cultures from immature and mature embryos of barley (Hordeum vulgare L.). Plant Cell Tissue Organ Cult 32: 19–25CrossRefGoogle Scholar
  19. Huang C-N, Wang J-H, Yan Q-S, Zhang X-Q, Yan Q-F (1995a) Plant regeneration from rice (Oryza sativa L.) embryogenic suspension cells cryopreserved by vitrification. Plant Cell Rep 14: 730–734CrossRefGoogle Scholar
  20. Huang C-N, Wang J-H, Yan Q-F, Yan Q-S, Zhang X-Q (1995b) Rapid establishment and cryopreservation of embryogenic cell suspension cultures of barley (Hordeum vulgare L.). Sci Agric Sin 28 (3): 21–27Google Scholar
  21. Jähne A, Lazzeri PA, Lörz H (1991) Regeneration of fertile plants from protoplasts derived from embryogenic cell suspensions of barley (Hordeum vulgare L.). Plant Cell Rep 10: 1–6CrossRefGoogle Scholar
  22. Kartha KK (ed) (1985) Cryopreservation of plant cells and organs. CRC Press, Boca Raton, 276 ppGoogle Scholar
  23. Kermode AR (1997) Approaches to elucidate the basis of desiccation-tolerance in seeds. Seed Sci Res 7 (2): 75–96CrossRefGoogle Scholar
  24. Kihara M, Funatsuki H (1995) Fertile plant regeneration from barley (Hordeum vulgare L.) protoplasts isolated from primary calluses. Plant Sci 106: 115–120CrossRefGoogle Scholar
  25. Langis R, Sohnabel B, Earle ED, Steponkus PL (1989) Cryopreservation of Brassica compestrist L. cell suspensions by vitrification. Cryo Lett 10: 421–428Google Scholar
  26. Laurie JD, Zhang G, McGann LE, Case DD (1996) Cryopreservation of maize immature embryos — embryo culture, cryopreservation, and effect of preculture conditions. In Vitro 32 (3, part II): 107A - 108AGoogle Scholar
  27. Lu B (1995) Diversity and conservation of the Triticeae genetic resources. Chin Biodiv 3 (2): 63–68Google Scholar
  28. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15: 473–497CrossRefGoogle Scholar
  29. Oka S, Saito N, Kawaguchi H (1995) Histological observations on initiation and morphogenesis in immature and mature embryo derived callus of barley (Hordeum vulgare L.). Ann Bot 76: 487–492CrossRefGoogle Scholar
  30. Portrykus I, Harms CT, Lörz H (1979) Callus formation from cell culture protoplasts of corn (Zea mays L.). Theor Appl Genet 54: 209–214CrossRefGoogle Scholar
  31. Reinhoud PJ, Versteege I, Van Iren F, Kijne JW (1997) Induction of leas in tobacco suspension cells contributes to their tolerance to vitrification. Cryo Lett 18: 69Google Scholar
  32. Ritala A, Aspegren K, Kurten U, Salmenkallio-Marttila M, Mannonen L, Hannus R, Kauppinen V, Teeri TH, Enari TM (1994) Fertile transgenic barley by particle bombardment of immature embryos. Plant Mol Biol 24: 317–325PubMedCrossRefGoogle Scholar
  33. Sakai A (1986) Cryopreservation of germplasm of woody plants. In: Bajaj YPS (ed) Biotechnol- ogy in agriculture and forestry, vol 1. Springer, Berlin Heidelberg New York, pp 113–129Google Scholar
  34. Sakai A, Kobayashi S, Oiyama I (1990) Cryopreservation of nucellar cells of navel orange (Citrus sinensis var. brasiliensis Tanaka) by vitrification. Plant Cell Rep 9: 30–33CrossRefGoogle Scholar
  35. Salmenkallio-Marttila M, Kaupinnen V (1995) Efficient regeneration of fertile plants from protoplasts isolated from microspore cultures of barley (Hordeum vulgare L.). Plant Cell Rep 14: 253–256CrossRefGoogle Scholar
  36. Singh RR, Kemp JA, Kollmorgen JF, Qureshi JA, Fincher GB (1997) Fertile plant regeneration from cell suspension and protoplast cultures of barley (Hordeum vulgare cv. Schooner ). Plant Cell Tissue Organ Cult 49: 121–127CrossRefGoogle Scholar
  37. Stanwood PC (1980) Tolerance of crop seeds to cooling and storage in liquid nitrogen (−196 °C). J Seed Technol 5: 26Google Scholar
  38. Stanwood PC (1985) Cryopreservation of seed germplasm for genetic conservation. In: Kartha KK (ed) Cryopreservation of plant cells and organs. CRC Press, Boca Raton, pp 199–226Google Scholar
  39. Steponkus PL (1985) Cryobiology of isolated protoplasts: application to plant cell cryopreservation. In: Kartha KK (ed) Cryopreservation of plant cells and organs. CRC Press, Boca Raton, pp 49–60Google Scholar
  40. Steponkus PL, Langis R, Fujikawa S (1992) Cryopreservation of plant tissues by vitrification. In: Steponkus PL (ed) Advances in low-temperature biology, vol 1. JAI Press, London, pp 1–61Google Scholar
  41. Stirn S, Mordhorst AP, Fuchs S, Lörz H (1995) Molecular and biochemical markers for embryo-genic potential and regenerative capacity of barley (Hordeum vulgare L.) cell cultures. Plant Sci 106: 195–206CrossRefGoogle Scholar
  42. Stöldt A, Wang X, Lörz H (1996) Primary callus as source of totipotent barley (Hordeum vulgare L.) protoplasts. Plant Cell Rep 16: 137–141CrossRefGoogle Scholar
  43. Takeuchi M, Matsushima H, Sugawara Y (1982) Totipotency and viability of protoplasts after longterm freeze preservation. In: Fujiwara A (ed) Proceedings of the 5th international congress of plant tissue and cell culture. Maruzen, Tokyo, Japan, pp 797–798Google Scholar
  44. Towill LE, Mazur P (1975) Studies on the reduction of 2,3,5-triphenyltetrazolium chloride as a viability assay for plant tissue cultures. Can J Bot 53: 1097–1102CrossRefGoogle Scholar
  45. Vasil V, Srivastava V, Castillo AM, Fromm ME, Vasil IK (1993) Rapid production of transgenic wheat plants by direct bombardment of cultured immature embryos. Bio/Technology 11: 1553–1558CrossRefGoogle Scholar
  46. von Bothmer R, Seherg O, Jacobsen N (1992) Genetic resources in the Triticeae. Hereditas 116: 141–150Google Scholar
  47. Wan Y, Lemaux PG (1994) Generation of large numbers of independently transformed fertile barley plants. Plant Physiol 104: 37–48PubMedGoogle Scholar
  48. Wang J-H, Huang C-N (1995) Histological studies on the dedifferentiation and redifferentiation pattern of barley (Hordeum vulgare L.) mature embryo cells. J Hangzhou University (Natural Science Edition) 22: 102–106Google Scholar
  49. Wang J-H, Huang C-N (1996) Application of antifreeze proteins in hypothermic preservation and cryopreservation. Chin J Cell Biol 18 (3): 107–111Google Scholar
  50. Wang J-H, Huang C-N (1998a) Assessment of antifreeze proteins and water deficit proteins during cryopreservation of Oryza sativa and Dendrobium candidum cells. Abstracts of XVIII international congress of genetics, 10–15 Aug, Beijing, 186 ppGoogle Scholar
  51. Wang J-H, Huang C-N (1998b) Progress on germplasm cryopreservation of woody plants. World For Res 11 (5): 6–11Google Scholar
  52. Wang J-H, Yan Q-F, Huang C-N (1996) Plant regeneration from barley (Hordeum vuglare L.) immature inflorescences cryopreserved by vitrification. Acta Bot Sin 38: 730–734Google Scholar
  53. Wang J-H, Ge J-G, Liu F, Huang C-N (1998) Ultrastructural changes during cryopreservation of rice (Oryza sativa L.) embryogenic suspension cells by vitrification. Cryo Lett 19: 49–54Google Scholar
  54. Wang J-H, Bian H-W, Huang C-N, Ge J-G (1999) Studies on the application of antifreeze proteins in cryopreservation of rice embryogenic suspension cells. Acta Biol Exp Sin 32: 271–276Google Scholar
  55. Wang XH, Lazzeri PA, Lörz H (1992) Chromosomal variation in dividing protoplasts derived from cell suspension of barley (Hordeum vulgare L.). Theor Appl Genet 85: 181–185Google Scholar
  56. Withers LA (1978) Freeze-preservation of cultured cells and tissues. In: Thorpe T (ed) Frontiers of plant tissue culture. Calgary University, Calgary, pp 297–306Google Scholar
  57. Withers LA (1982) The development of cryopreservation techniques for plant cell, tissue and organ culture. In: Fujiwara A (ed) Plant tissue culture. Tokyo, Japan, pp 793–794Google Scholar
  58. Withers LA (1987) Long-term preservation of plant cells, tissues and organs. Oxf Sury Plant Mol Cell Biol 4: 221–272Google Scholar
  59. Yan Q-S, Zhang X-Q, Shi J-B, Li J-M (1991) Green plant regeneration from protoplasts of barley (Hordeum vulgare L.). Chin Sci Bull 36: 932–935Google Scholar
  60. Yan Q-F, Wang J-H, Huang C-N, Yan Q-S, Zhang X-Q (1994) Studies on cryopreservation of rice (Oryza sativa L.) suspension cultures. Acta Biol Exp Sin 27: 399–409Google Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2002

Authors and Affiliations

  • Jun-Hui Wang
    • 1
  • Chun-Nong Huang
    • 1
  1. 1.College of Life ScienceZhejiang UniversityHangzhouChina

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