The term “miniantibodies” describes artificial multivalent or multispecific recombinant antibody fragments. They resemble natural antibodies in carrying two or four binding sites with a flexible arrangement and a long “wing span” but they are much smaller, as they consist only of fusions of a scFv to an oligomerization module. Applications of multivalent and bispecific antibody fragments in a variety of formats have been reviewed (Plückthun and Pack 1997; Carter and Merchant 1997). Approaches to quantify the avidity gain achieved by the multivalency effect present in all the described miniantibody models have been reported (Crothers and Metzger 1972, Plückthun and Pack 1997, Müller et al. 1998b).
KeywordsCrystallization Fermentation Cysteine Oligomerization Polypeptide
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