Affinity Measurements by Band Shift and Competition ELISA

Part of the Springer Lab Manuals book series (SLM)


The affinity constant (see Neri, Montigiani et al. 1996 for a simple introduction) is a useful parameter for the characterisation of antibody-antigen interactions. This constant can be seen as a “thermometre” which allows us to answer the following question: at certain conditions of concentration, to which extent does antibody A bind to its antigen B?


Band Shift Recombinant Antibody Affinity Measurement Titre Plate Competition ELISA 
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Copyright information

© Springer-Verlag Berlin Heidelberg 2001

Authors and Affiliations

  1. 1.Institut für Pharmazeutische WissenschaftenETH ZürichZürichSwitzerland

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