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Affinity Measurements by Band Shift and Competition ELISA

Part of the Springer Lab Manuals book series (SLM)

Abstract

The affinity constant (see Neri, Montigiani et al. 1996 for a simple introduction) is a useful parameter for the characterisation of antibody-antigen interactions. This constant can be seen as a “thermometre” which allows us to answer the following question: at certain conditions of concentration, to which extent does antibody A bind to its antigen B?

Keywords

Band Shift Recombinant Antibody Affinity Measurement Titre Plate Competition ELISA 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

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Copyright information

© Springer-Verlag Berlin Heidelberg 2001

Authors and Affiliations

  1. 1.Institut für Pharmazeutische WissenschaftenETH ZürichZürichSwitzerland

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