Selecting Intracellular Antibodies Using the Two-Hybrid System

Part of the Springer Lab Manuals book series (SLM)


The ectopic expression of functional antibodies can be utilized to specifically interfere with the function of selected antigens in cells, tissues or in organisms [1]. The efficacy of the ectopic antibody expression has been already demonstrated in several cases and its use has potential applications to disease therapy and in functional genomic studies.


scFv Fragment Bait Plasmid scFv Library Intracellular Antibody Intact Yeast Cell 
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Copyright information

© Springer-Verlag Berlin Heidelberg 2001

Authors and Affiliations

  1. 1.Neuroscience ProgrammeInternational School for Advanced Studies (SISSA)TriesteItaly

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