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Selecting Intracellular Antibodies Using the Two-Hybrid System

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Antibody Engineering

Part of the book series: Springer Lab Manuals ((SLM))

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Abstract

The ectopic expression of functional antibodies can be utilized to specifically interfere with the function of selected antigens in cells, tissues or in organisms [1]. The efficacy of the ectopic antibody expression has been already demonstrated in several cases and its use has potential applications to disease therapy and in functional genomic studies.

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References

  1. Cattaneo, A. and S. Biocca, Intracellular Antibodies: Development and Applications, ed. Springer. 1997.

    Book  Google Scholar 

  2. Biocca, S., M.S. Neuberger, and A. Cattaneo, Expression and targeting of intracellular antibodies in mammalian cells. Embo J, 1990. 9(1): p. 101–8.

    PubMed  CAS  Google Scholar 

  3. Biocca, S. and A. Cattaneo, Intracellular immunization: antibody targeting to subcellular compartments. Trends Cell Biol, 1995. 5: p. 248–252.

    Article  PubMed  CAS  Google Scholar 

  4. Gilbert, H.F., Molecular and cellular aspects of thiol-disulfide exchange. Adv Enzymol Relat Areas Mol Biol, 1990. 63: p. 69–172.

    PubMed  CAS  Google Scholar 

  5. Biocca, S. et al., Redox state of single chain Fv fragments targeted to the endoplasmic reticulum, cytosol and mitochondria. Biotechnology (N Y), 1995. 13(10): p. 1110–5.

    Article  CAS  Google Scholar 

  6. Proba, K. et al., Antibody scFv fragments without disulfide bonds made by molecular evolution. J Mol Biol, 1998. 275(2): p. 245–53.

    Article  PubMed  CAS  Google Scholar 

  7. Visintin, M. et al., Selection of antibodies for intracellular function using a twohybrid in vivo system. Proocedings of the National Academy of Sciences USA, in press.

    Google Scholar 

  8. Hollenberg, S.M. et al., Identification of a new family of tissue -specific basic helixloop-helix proteins with a two-hybrid system. Molecular and Cellular Biology, 1995. 15(7): p. 3813–3822.

    PubMed  CAS  Google Scholar 

  9. Heidmann, S. et al., Flexibility and interchangeability of polyadenylation signals in Saccharomyces cerevisiae. Mol Cell Biol, 1994. 14(7): p. 4633–42.

    PubMed  CAS  Google Scholar 

  10. Wahle, E. and W. Keller, The biochemistry of 3′-end cleavage and polyadenylation of messenger RNA precursors. Annu Rev Biochem, 1992. 61: p. 419–40.

    Article  PubMed  CAS  Google Scholar 

  11. Wahle, E., The end of the message: 3′-end processing leading to polyadenylated messenger RNA. Bioessays, 1992. 14(2): p. 113–8.

    Article  PubMed  CAS  Google Scholar 

  12. Gietz, D. et al., Improved method for high efficiency transformation of intact yeast cells. Nucleic Acids Res, 1992. 20(6): p. 1425.

    Article  PubMed  CAS  Google Scholar 

  13. Hill, J. et al., DMSO-enhanced whole cell yeast transformation [published erratum appears in Nucleic Acids Res 1991 Dec 11;19(23):6688]. Nucleic Acids Res, 1991. 19(20): p. 5791.

    Article  PubMed  CAS  Google Scholar 

  14. Schiestl, R.H. and R.D. Gietz, High efficiency transformation of intact yeast cells using single stranded nucleic acids as a carrier. Curr Genet, 1989. 16(5–6): p. 339–46.

    Article  PubMed  CAS  Google Scholar 

  15. Breeden, L. and K. Nasmyth, Regulation of the yeast HO gene. Cold Spring Harb Symp Quant Biol, 1985. 50: p. 643–50.

    Article  PubMed  CAS  Google Scholar 

  16. Bartel, P. et al., Elimination of false positives that arise in using the two-hybrid system, in Biotechniques. 1993. p. 920–4.

    Google Scholar 

  17. Evan, G.I. et al., Isolation of monoclonal antibodies specific for human c-myc proto- oncogene product. Mol Cell Biol, 1985. 5(12): p. 3610–6.

    PubMed  CAS  Google Scholar 

  18. McCafferty, J. et al., Selection and rapid purification of murine antibody fragments that bind a transition-state analog by phage display. Appl Biochem Biotechnol, 1994. 47(2–3): p. 157–71; discussion 171–3.

    Article  PubMed  CAS  Google Scholar 

  19. Griffiths, A.D. et al., Isolation of high affinity human antibodies directly from large synthetic repertoires. Embo J, 1994. 13(14): p. 3245–60.

    PubMed  CAS  Google Scholar 

  20. Hoogenboorn, H.R. et al., Multi-subunit proteins on the surface of filamentous phage: methodologies for displaying antibody (Fab) heavy and light chains. Nucleic Acids Res, 1991. 19(15): p. 4133–7.

    Article  Google Scholar 

  21. Persic, L. et al., Targeting vectors for intracellular immunisation. Gene, 1997. 187(1): p. 1–8.

    Article  PubMed  CAS  Google Scholar 

  22. Bartel, P.L. et al., Using the two-hybrid system to detect protein-protein interaction, in Cellular interaction in development: a practical approach, D.A. Hartley, Editor. 1993, IRL Press: Oxford. p. 153–179.

    Google Scholar 

  23. Vaughan, T.J. et al., Human antibodies with sub-nanomolar affinities isolated from a large non-immunized phage display library [see comments]. Nat Biotechnol, 1996. 14(3): p. 309–14.

    Article  PubMed  CAS  Google Scholar 

  24. Sheets, M.D. et al., Efficient construction of a large nonimmune phage antibody library: the production of high-affinity human single-chain antibodies to protein antigens [published erratum appears in Proc Natl Acad Sci USA 1999 Jan 19;96(2):795]. Proc Natl Acad Sci USA, 1998. 95(11): p. 6157–62.

    Article  PubMed  CAS  Google Scholar 

  25. Cardinale, A. et al., The mode of action of Y13–259 scFv fragment intracellularly expressed in mammalian cells. FEBS Lett, 1998. 439(3): p. 197–202.

    Article  PubMed  CAS  Google Scholar 

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Correspondence to Antonino Cattaneo .

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© 2001 Springer-Verlag Berlin Heidelberg

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Visintin, M., Cattaneo, A. (2001). Selecting Intracellular Antibodies Using the Two-Hybrid System. In: Kontermann, R., Dübel, S. (eds) Antibody Engineering. Springer Lab Manuals. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-04605-0_15

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  • DOI: https://doi.org/10.1007/978-3-662-04605-0_15

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-540-41354-7

  • Online ISBN: 978-3-662-04605-0

  • eBook Packages: Springer Book Archive

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