Use of Polyclonal Avian Antibodies

  • Ingrid Behn
  • Undine Hommel
  • Michael Erhard
  • Andreas Hlinak
  • Rüdiger Schade
  • Christine Schwarzkopf
  • Christian Staak
Part of the Springer Lab Manuals book series (SLM)

Abstract

for many years antibodies have been used for quantitation and detection of a lot of different substances. The specific recognition between antibodies and antigens has led to the development of diverse methods for basic research in the sciences as well as for diagnosis in human and veterinary medicine.

Keywords

Agar Titration Turkey Myeloma Fibrinogen 

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References

  1. Akita EM, Nakai S (1993) Comparison of four purification methods for the production of immunoglobulins from eggs laid by hens immunized with enterotoxigenic E. coli strain. J. Immunol. Methods 160:207–214CrossRefGoogle Scholar
  2. Ambrosius H, Luppa, H. (1987) Immunhistochemie, Grundlagen und Techniken, Akademie-Verlag Berlin, pp. 105–140.Google Scholar
  3. Behn E (1991) Immunodiffusion. In: Friemel H (ed) Immunologische Arbeitsmethoden, 4. Auflage, Gustav Fischer Verlag, Jena, pp. 81–95Google Scholar
  4. Behn I, Hommel U, Oertel M, Hauschildt S (1996) Kinetics of IgY Formation after Immunisation of Hens with different Protein Antigens. Altex 13 Supplement 96:18–21Google Scholar
  5. Benedict AA, Yamaga K (1976) Immunoglobulins and Antibody Production in Avian Species, In: Marchalonis JJ (ed) Comparative Immunology, Blackwell Scient. Publ., Oxford London Edinburgh Australia.Google Scholar
  6. Bjerrum OJ, Schaffner-Nielsen C (1986) Buffer systems and transfer parameters for semidry electroblotting with horizontal apparatus. Electro-phoresis 86:315–319Google Scholar
  7. Brumfield HP, Benson H, Pomeroy BS (1961) Procedure for Modified Complement Fixation Test with Turkey, Duck, and Chicken Serum Antibody. Avian Dis 5:270–282CrossRefGoogle Scholar
  8. Clarke HGM, Freeman T (1966) A quantitative immuno-electrophorasis method (Laurell electrophoresis). Protides Biol Fluids proc Colloq 14:503–509Google Scholar
  9. Concetti A, Ripani E, Barboni L, Torregiani E, Bombardelli E, Gariboldi P, Venanz FM (1994) Immunorecognition of ring skeleton of taxanes by chicken yolk antibodies. Bilogical Chemistry Hoppe-Seyler 375(6):419–423CrossRefGoogle Scholar
  10. Cuelo AC (1983) Immunohistochemistry. IBRO Handbook Series: Methods in the Neurosciences Vol. 3, John Wiley amp; Sons, Chichester New York Brisbane Toronto SingaporeGoogle Scholar
  11. Danielpour D (1993) Improved sandwich enzyme-linked immunosorbent assays for transforming growth factor beta1. J Immunol Meth 158:17–25CrossRefGoogle Scholar
  12. Engvall E (1980) Enzyme Immunoassay ELISA and EMIT. Meth Enzymol 70:419Google Scholar
  13. Erhard MH, Bergmann J, Renner M, Hofmann A, Heinritzi K (1996) Prophylaktische Wirkung von spezifischen Dotterantikörpern bei Escherichia coli K88 (F4)-bedingten Durchfallerkrankungen von Absatzferkeln. (Prophylactic effect of specific egg yolk antibodies in diarrhea of weaned piglets cause by Escherichia coli K88 (F4)). Vet Med A 43:217–223CrossRefGoogle Scholar
  14. Erhard MH, Kellner J, Eichelberger J, Lösch U (1993) Neue Möglichkeiten in der oralen Immunprophylaxe des Kalbes-ein Feldversuch mit spezifischen Eiantikörpern (New aspects in oral immunprophylaxis for the prevention of infectious diarrhea of newborn calves-a field study with specific antibodies). Berl Münch Tierärztl Wschr 106:383–387Google Scholar
  15. Erhard MH, Quistorp I von, Schranner I, Jüngling A, Kaspers B, Schmidt P, Kühlmann R (1992) Development of specific enzyme linked immunosorbent antibody assay systems for the detection of chicken immuno-globulins G, M, and A using monoclonal antibodies. Poultry Sci 71:302–310CrossRefGoogle Scholar
  16. Erhard MH, Schmidt P, Hofmann A, Bergmann J, Mittermeier P, Kaufmann P, Wiesmüller K-H, Bessler W, Lösch U (1997) The lipopeptid Pam3Cys-Ser-(Lys)4: an alternative aative adjuvant to Freund’s adjuvant for immunisation of chicken to produce egg yolk antibodies. ATLA 25:173–181Google Scholar
  17. Ey PL, Prowse SJ, Jenkin CR (1978) Isolation of pure IgGl, IgG2a and IgG2b immunoglobulins from mouse serum using protein-A sepharose. Immunochem 15:429–457CrossRefGoogle Scholar
  18. Fahey JL and McKelvey EM (1965). Quantitative determination of serum immunoglobulins in antibody-agar plates. J Immunol 94:84–91.Google Scholar
  19. Ferencik M. (1993). Handbook of Immunochemistry, Chapman and Hall, London Glasgow New York Tokyo Melbourne Madras. pp. 312–386.CrossRefGoogle Scholar
  20. Friemel H (1991) Immunelektrophorese. In: Friemel H (ed) Immunologische Arbeitsmethoden, 4. Auflage, Gustav Fischer Verlag, Jena, pp. 96–107Google Scholar
  21. Goding JW (1976) Conjugation of antibodies with fluorochromes: modifications to the standard methods. J Immunol Meth 13:215–226CrossRefGoogle Scholar
  22. Guiterrez JA, Guirriero V (1991) Quantitation of Hsp 70 in tissues using a competitive enzyme-linked immunosorbent assay. J Immunol. Methods 143:81–88CrossRefGoogle Scholar
  23. Grabar C, Williams CA (1953) A method permitting the combined study of the electrophoretic and immunochemical properties of a mixture of proteins: application to blood serum. Biophys Acta 10:193–194CrossRefGoogle Scholar
  24. Hlinak A, Schrödl W, Witt S, Schade R, Krüger M, (1996) Production of Egg Yolk Antibodies against Human Cell-Associated Antigens Altex 13 Supplement 96:76–79Google Scholar
  25. Ikemori Y, Ohta M, Umeda K, Peralta RC, Kuroki M, Yokoyama H, Kodama Y (1996) Passage of chicken egg yolk antibody treated with hydroxypropyl methylcellulose phtalate in the gastrointestinal tract of calves. J Vet Med Sci 58(4):365–367CrossRefGoogle Scholar
  26. Ikemori Y, Ohta M, Umeda K, Icario Jr. FC, Kuroki M, Yokoyama H, Kodama Y (1997) Passive protection of neonatal calves against bovine coronavirus-induced diarrhea by administration of egg yolk or colostrum antibody powder. Vet. Microbiol. 58:105–111CrossRefGoogle Scholar
  27. Kellner J, Erhard MH, Lösch U (1994) Therapeutischer Einsatz von spezifischen Eiantikörpern bei Säugferkeldurchfall-ein Feldversuch. Tierärztl Umschau 49:31–34Google Scholar
  28. Kowalczyk K, Daiss J, Halpern J, Roth TF (1985) Quantification of maternal-fetal IgG transport in the chicken. Immunol 54:755–762Google Scholar
  29. Kronick MN (1986) The use of phycobiliproteins as fluorescent labels in immunoassay J Immunol Meth 92:1–13CrossRefGoogle Scholar
  30. Kyhse-Anderson J (1984) Electroblotting of multiple gels: a simple apparatus without buffer tank for rapid transfer of proteins from polyacrylamide to nitrocellulose. J Biochem Biophys Meth 10:203–209CrossRefGoogle Scholar
  31. Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:655–685CrossRefGoogle Scholar
  32. Lanham SM, Godfrey DG (1970) Isolation of salivarian trypanosomes from man and other mammals using DEAE-cellulose. Exp Parasitol 28:521–534CrossRefGoogle Scholar
  33. Larsson A, Sjoquist J (1990) Chicken IgY. Utilizing the evolutionary difference. Comp Immun Microbiol Infect Dis 13(4):199–201CrossRefGoogle Scholar
  34. Laurell CB (1965) Antigen-antibody crossed electrophoresis. Anal Biochem 10:358–372CrossRefGoogle Scholar
  35. Laurell CB (1966) Quantitative estimation of proteins by electrophoresis in agarose gel containing antibodies. Anal Biochem 15:45–52CrossRefGoogle Scholar
  36. Lösch U, Schranner I, Wanke R, Jürgens L (1986) The chicken egg, an anti-body source. J Vet Med B 98:609–619CrossRefGoogle Scholar
  37. Mancini G, Carbonara AO, Heremans JF (1965) Immunochemical quantitation of antigens by simple radial immunodiffusion. Immunochemistry 2:235–254CrossRefGoogle Scholar
  38. Marquart WW, Newman JA (1970) A direct complement-fixation test for detection of mycoplasma antibodies in chicken serum. Avian Dis 15, 139–149CrossRefGoogle Scholar
  39. Mohr J (1991) Markierung von Antikörpern mit Fluorochromen, In: Friemel H (ed) Immunologische Arbeitsmethoden, Gustav Fischer Verlag, Jena, pp. 135–188Google Scholar
  40. Montes Perez RC, Murcia Meija C, Zarco Quintero L (1994) Production of antibodies against progesterone from the egg yolk of hens and from rabbit blood serum to be used in radioimmunoassay. Veterinaria-Mexico 25(2):117–125Google Scholar
  41. Morino P, Herrera-Marschitz M, Castel MN, Ungerstedt U, Varro A, Dockray G, Hökfelt T (1994). Cholecystokinin in cortico-striatal neurones in the rat: Immunohistochemical studies at the light and microscopical level. Eur J Neurosci 6:681–692CrossRefGoogle Scholar
  42. Nakane PK, Kawoi A (1974) Peroxidase antibody. A new method of conjugation. J Histochem Cytochem 22:1084–1090CrossRefGoogle Scholar
  43. Ntakarutimana V, Demedts P, van Sande M, Scharpè S (1992) A simple and economical strategy for downstream processing of specific antibodies to human transferrin from egg yolk. J Immunol Methods 153:133–140CrossRefGoogle Scholar
  44. O.I.E. (Office International des Epizooties)“Manual of Standards for Diagnostic Tests and Vaccines“, Paris 1996Google Scholar
  45. Özpinar H, Erhard MH, Aytug N, Özpinar A, Baklaci C, Karamüptüglu S, Hofmann A, Lösch U (1996) Dose dependent effects of specific egg-yolk antibodies on diarrhoea of newborn calves. Prev Vet Med 27:67–73CrossRefGoogle Scholar
  46. Peralta RC, Yokoyama H, Ikemori Y, Kuroki M, Kodarna Y (1994) Passive immunisation against experimental salmonellosis in mice by orally administered hen egg-yolk antibodies specific for 14-kDa fimbriae of Salmonella enderitidis. J Med Microbiol 41(l):29–35CrossRefGoogle Scholar
  47. Poison A, von Wechmar MB, Fazakerley G (1980) Antibodies to protein from yolk of immunized hens. Immunol Commun 9:495–514Google Scholar
  48. Porstmann T, Porstmann B (1991) Enzymimmunoassay. In: Friemel H (ed) Immunologische Arbeitsmethoden, Gustav Fischer Verlag, Jena, pp. 135–188Google Scholar
  49. Reisher JI, Orr HC (1968) Removal of fluorescein isothiocyanate from sephadex after filtration of conjugated proteins. Anal Biochem 26:178–179.CrossRefGoogle Scholar
  50. Ressler N (1960) Two-dimensional electrophoresis of serum protein antigens in an antibody containing buffer. Clin Chim Acta 5:795–803CrossRefGoogle Scholar
  51. Rieger A, Bürger W, Hiepe W, Schade R (1996) Determination of human serum CRP using a chicken egg yolk antibody (IgY) to avoid interferences with rheumatoid factors. Comparison with mammalian antibodies. ALTEX Suppl. 13:57–61Google Scholar
  52. Rose ME, Orlan E, Buttress N (1974) Immunoglobulin classes in the hens egg: their segregation in yolk and white. Eur J Immunol 4:521–523CrossRefGoogle Scholar
  53. Sakamoto N, Takatsuji K, Shiosaka S, Tateishi K, Hashimura E, Miura S, Hamaoka T, Tohyama M (1984). Cholecystokinin-8-like immunoreactivity in the pre-and post-central gyri of the human cerebral cortex. Brain Res 307:77–83CrossRefGoogle Scholar
  54. Schade R, Oelβner W, Göhler K, Ott T, Pfister C, Henklein P, Furkert, FJ, Rupprich Ch, Fekete M, Penke B, Jojart, I (1988) Characterization of anti CCK-8 antibodies raised in hens and rabbits by means of several in vitro and in vivo animal models. Biogenic Amines 5:535–550Google Scholar
  55. Schade R, Henklein P, Hlinak A, de Vente J, Steinbusch H (1996) Specificity of chicken (IgY) versus rabbit (IgG) antibodies raised against cholecystokinin octapeptide (CCK). Altex 13 Supplement 96:80–85Google Scholar
  56. Schade R., Pfister C, Halatsch R., Henklein P. (1991) Polyclonal IgY antibodies from chicken egg yolk-an alternative to the production of mammalian IgG type antibodies in rabbits. ATLA 19:403–419.Google Scholar
  57. Schmidt P, Wiedemann V, Kühlmann R, Linckh E, Lösch U (1989) Chicken egg antibodies for prophylaxis and therapy of infectious intestinal diseases. II. In vitro studies on gastric and enteric digestion of egg yolk antibodies specific against pathogenic Escherichia coli strains. J Vet Med B 36:619–628CrossRefGoogle Scholar
  58. Schniering A (1995) Untersuchungen zum Koproantigen-Nachweis des Intestinalparasiten Ascaris suum unter Verwendung von aviären vitellinen Antikörpern. Inaugural-Dissertation, Fachbereich Veterinärmedizin der Freien Universität Berlin (Journal Nr. 1837), GermanyGoogle Scholar
  59. Schniering A, Schade R, Hiepe T (1996) Aufbau eines Assays zu Detektion von Ascaris suum-Antigenen auf der Basis von IgY. ALTEX Suppl. 13:62–65Google Scholar
  60. Shimizu M, Fitzsimmons RC, Nakai S (1988) Anti-E. coli immunoglobulin Y isolated from egg yolk of immunized chickens as a potential food ingredient. J Food Sci 53:1360–1366CrossRefGoogle Scholar
  61. Shimizu M, Nagashima H, Sano K, Hashimoto K, Ozeki M, Tsuda K, Hatta H (1992) Molecular stability of chicken and rabbit immunoglobulin. Biosc Biotech Biochem 56:270–274CrossRefGoogle Scholar
  62. Shimizu M, Watanabe A, Tanak A (1995) Detection of high molecular weight mucin-like glcoprotein-A (HMGP-A) of human milk by chicken egg yolk antibody. Biosci Biotech Biochem 59(1):138–139CrossRefGoogle Scholar
  63. Shimizu M, Nagashima H, Hashimotot K, Suzuki T (1994) Egg-yolk antibody (IgY) stability in aqueous solution with high sugar concentrations. J Food Sci 59(4):763CrossRefGoogle Scholar
  64. Sofroniew MV (1983) Golgi-like immunoperoxidase staining of neurons producing specific substances or of neurons transporting exogenous tracer proteins. In: Cuello AC (ed) Immunohistochemistry John Wiley and Sons, Chichester, New York, Brisbane Toronto, Singapore, pp. 413–417.Google Scholar
  65. Stolfi RL, Fugmann RA, Jensen JJ, Sigel MM (1971) A Cl Fixation Method for the Measurement of Chicken anti-Viral Antibody. Immunology 20:299–306Google Scholar
  66. Tjissen P (1985) “Laboratory Techniques in Biochemistry and Molecular Biology: Practice and Theory of Enzyme Immunoassays” Elsevier, Amsterdam, New York, OxfordGoogle Scholar
  67. Towbin H, Gordon J (1984) Immunoblotting and dot immunobinding-cur-rent status and outlook. J Immunol Meth 72:313–340CrossRefGoogle Scholar
  68. Towbin H, Staehelm T, Gordon J (1979) Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets procedure and some applications Proc Natl Acad Sci 76:4259–4354CrossRefGoogle Scholar
  69. Van Regenmortel, Burckhard J (1985) Quantitative Microcomplement Fixation tests using Chicken anti-Viral Antibody Extracted from Egg Yolk. J Virol Meth 11:217–223CrossRefGoogle Scholar
  70. Vick K (1995) Circadiane Rhythmik von Cholecystokinin und Dopamin im mesolimbischen und nigrostriatalen System der Ratte sowie deren Einfluβ auf pharmakologisch induzierte Gehaltsänderungen, Humboldt University, Berlin: Inaugural DissertationGoogle Scholar
  71. Wiedemann V, Kühlmann R, Schmidt P, Erhardt W, Lösch U (1990) Chicken egg antibodies for prophylaxis and therapy of infectious intestinal diseases. III. In vivo tenacity test in piglets with artificial jejunal fistula. J Vet Med B 37:283–291CrossRefGoogle Scholar
  72. Yokoyama H, Peralta RC, Sendo S, Ikemori Y, Kodarna Y (1992) Detection of passage and absorption of chicken egg yolk immunoglobulins in the gastrointestinal tract of pigs by use of enzyme-linked immunosorbent assay and fluorescent antibody testing. Am J Vet Res 54:867–872Google Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2001

Authors and Affiliations

  • Ingrid Behn
    • 1
  • Undine Hommel
    • 1
  • Michael Erhard
    • 2
  • Andreas Hlinak
    • 3
  • Rüdiger Schade
    • 3
  • Christine Schwarzkopf
    • 4
  • Christian Staak
    • 5
  1. 1.Pharmazie und PsychologieUniversität LeipzigLeipzigGermany
  2. 2.Veterinärmedizinische Fakultät, Veterinär-Physiologisches InstitutUniversität LeipzigLeipzigGermany
  3. 3.Institut für Pharmakologie und ToxikologieUniversitätsklinikum Charité der Humboldt-UniversitätBerlinGermany
  4. 4.Zentrales Institut des Sanitätsdienstes der Bundeswehr KoblenzLaborabt. II (Vet. Med)MainzGermany
  5. 5.Bundesinstitut für gesundheitlichen Verbraucherschutz und VeterinärmedizinBerlinGermany

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