Electrotransformation of Listeria species
Electroporation offers an efficient system for the introduction of genetic material into listeriae. Conjugation and protoplast transformation of Listeria monocytogenes has been reported (Flamm et al. 1984, Vicente et al. 1987). However, transformation rates (∼ 103/ μg of DNA) produced by these methods are too low for use in many molecular techniques. To facilitate the entry of DNA by electroporation, sufficient intensity of current must be applied to produce the optimum number of permeablilized areas. The intensity of current is a function of the field strength and time constant used.
KeywordsSucrose Magnesium Agar Penicillin Bacillus
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