Skip to main content

Electrotransformation of Salmonella typhimurium

  • Chapter
Electrotransformation of Bacteria

Part of the book series: Springer Lab Manuals ((SLM))

  • 1143 Accesses

Abstract

No natural competence is known in Salmonella. Results in the 70’s showed that Ca2+ shock method was able to give transformation but at very low frequency. With this method, a clear cut dependence on the cell envelope composition was observed. These two draw backs are not present when bacteria are electrotransformed. Electrotransformation appears as a quick and easy to use procedure.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 129.00
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 169.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Reference

  • Binotto, J., P. R. MacLachlan and K. E. Sanderson. 1991. Electrotransforma- tion in Salmonella typhimurium LT2. Can J Microbiol. 37: 474–477.

    Article  CAS  Google Scholar 

  • Eynard, N., S. Sixou, N. Duran and J. Teissie. 1992. Fast kinetics studies of Escherichia coli electrotransformation. Eur J Biochem. 209: 431–436.

    Article  CAS  Google Scholar 

  • O’Callaghan, D. and A. Charbit. 1990. High efficiency transformation of Salmonella typhimurium and Salmonella typhi by electroporation. Mol Gen Genet. 223: 156–158.

    Article  Google Scholar 

  • Sixou, S., N. Eynard, J. M. Escoubas, E. Werner and J. Teissie. 1991. Optimized conditions for electrotransformation of bacteria are related to the extent of electropermeabilization. Biochim Biophys Acta. 1088: 135–138.

    Google Scholar 

  • Taketo, A. 1988. DNA transfection of Escherichia coli by electroporation. Biochim Biophys Acta. 949: 318–324.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 2000 Springer-Verlag Berlin Heidelberg

About this chapter

Cite this chapter

Eynard, N. (2000). Electrotransformation of Salmonella typhimurium . In: Eynard, N., Teissié, J. (eds) Electrotransformation of Bacteria. Springer Lab Manuals. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-04305-9_7

Download citation

  • DOI: https://doi.org/10.1007/978-3-662-04305-9_7

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-08593-2

  • Online ISBN: 978-3-662-04305-9

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics