Introduction of Plasmids into Azospirillum brasilense by Electroporation

  • Ann Vande Broek
  • Jos Vanderleyden
Part of the Springer Lab Manuals book series (SLM)


The genus Azospirillum comprises Gram N2 fixing soil bacteria living in close association with the roots of numerous plants. Field trials, carried out at different locations have demonstrated significant plant growth promotion upon Azospirillum inoculation. Based on 16S rRNA similarity, the genus Azospirillum has been classified within the alpha subdivision of Proteobacteria. Plasmids of the IncP group are stably maintained in a wide range of Azospirillum strains and can be succesfully transferred to Azospirillum either by conjugation or by electroporation. As compared to conjugation, the electroporation technique offers the advantages of being much faster and avoiding problems with counterselection of the Escherichia coli donor strain.


Azospirillum Brasilense Electroporation Cuvette Azospirillum Strain Pulser Electroporation IncP Plasmid 
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Copyright information

© Springer-Verlag Berlin Heidelberg 2000

Authors and Affiliations

  • Ann Vande Broek
    • 1
  • Jos Vanderleyden
    • 1
  1. 1.F.A. Janssens Laboratory of GeneticsHeverleeBelgium

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