Abstract
E. coli is the most popular bacteria in laboratories of bacterial genetics and biotechnology. Getting DNA transfer and expression in such a system is required in many experiments. Electropulsation is now known as being a highly efficient approach. Its use is very easy (just mix plasmid and bacteria, then pulse) and not time consuming. Very simple electropulsators can be used (E. coli pulser). There is no need to induce a wall modification in the case of E. coli. No major improvements are found after EDTA and lysozyme treatment. Most manufacturers provide protocols for E. coli electrotransformations, which can be downloaded from the WEB.
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References
Chuang, S. E., A. L. Chen and C. C. Chao. 1995. Growth of E. coli at low temperature dramatically increases the transformation frequency by electroporation. Nucleic Acids Res. 23: 1641
Davis, F. G., M. D. Dibner and J. F. Battey. 1986. Basic methods in molecular biology in Elsevier Science, Amsterdam
Eynard, N., S. Sixou, N. Duran and J. Teissie. 1992. Fast kinetics studies of Escherichia coli electrotransformation. Eur J Biochem. 209: 431–436
Sharma, R. C. and R. T. Schimke. 1996. Preparation of electrocompetent E. coli using salt-free growth medium. Biotechniques. 20: 42–44
Sheng, Y., V. Mancino and B. Birren. 1995. Transformation of Escherichia coli with large DNA molecules by electroporation. Nucleic Acids Res. 23: 1990–1996
Sixou, S., N. Eynard, J. M. Escoubas, E. Werner and J. Teissie. 1991. Opti-mized conditions for electrotransformation of bacteria are related to the extent of electropermeabilization. Biochim Biophys Acta. 1088: 135–138
Tung, W. L. and K. C. Chow. 1995. A modified medium for efficient electrotransformation of E. coli. Trends Genet. 11: 128–129
Xie, T. D., L. Sun, H. G. Zhao, J. A. Fuchs and T. Y. Tsong. 1992. Study of mechanisms of electric field-induced DNA transfection. IV. Effects of DNA topology on cell uptake and transfection efficiency. Biophys J. 63: 1026–1031
Xie, T. D. and T. Y. Tsong. 1992. Study of mechanisms of electric field-induced DNA transfection. III. Electric parameters and other conditions for effective transfection. Biophys J. 63: 28–34
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© 2000 Springer-Verlag Berlin Heidelberg
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Eynard, N., Teissié, J. (2000). Electrotransformation of Escherichia coli . In: Eynard, N., Teissié, J. (eds) Electrotransformation of Bacteria. Springer Lab Manuals. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-04305-9_3
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DOI: https://doi.org/10.1007/978-3-662-04305-9_3
Publisher Name: Springer, Berlin, Heidelberg
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