Electroporation of Legionella Species
The molecular analysis of Legionella pneumophila had been limited by the lack of methods to genetically manipulate the organism. Since bacteriophages and transduction have not been reported in L. pneumophila, methods of introducing DNA into the organism were limited to conjugation until 1990. Since then electroporation has rapidly replaced conjugation as the preferred method of introducing DNA into L. pneumophila for mutagenesis — mediated by transposons (Pope et al, 1994, Wiater et al, 1994, Pruckler et al, 1995, McClain and Engleberg, 1996, Hickey and Cianciotto, 1997) or by allelic exchange (Cianciotto and Fields, 1992, Sadosky et al, 1993, Abu Kwaik et al, 1997) and for complementation studies (Marra and Shuman, 1992, Berger et al, 1994, Abu Kwaik and Pederson, 1996).
KeywordsAllelic Exchange Electroporation Cuvette Buffer Charcoal Yeast Extract Ferric Pyrophosphate Electroporation Apparatus
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