Abstract
The basic principle of electrotransformation is to apply electric pulses to a mixture of bacteria and plasmids in an aqueous solution. In most protocols, only one pulse is needed. This is obtained by applying a selected voltage U (volts) on two electrodes, the interelectrode space being filled with the mixture. In all experiments dealing with gene transfer in bacteria, the two electrodes are flat and parallel with a width d (cm). As a consequence, a field E (V/cm) is present between the two electrodes with a strength:
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References
Eynard N., Sixou S., Duran N. and Teissie J. (1992) Fast kinetics studies of E.coli electrotransformation Eur. J. Biochem; 209, 431 - 436
Loomis-Husselbee J.W., Cullen P.J., Irvine R.F. and Dawson A.P. (1991) Electroporation can cause artefacts due to solubilization of cations from the electrode plates Biochem. J. 277, 883 - 885
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© 2000 Springer-Verlag Berlin Heidelberg
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Teissié, J. (2000). A Critical Introduction to the Technology of Bacterial Electrotransformation. In: Eynard, N., Teissié, J. (eds) Electrotransformation of Bacteria. Springer Lab Manuals. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-04305-9_2
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DOI: https://doi.org/10.1007/978-3-662-04305-9_2
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-08593-2
Online ISBN: 978-3-662-04305-9
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