Electrotransformation of Yersinia ruckeri
Electroporation may be used for transferring macromolecules (proteins and nucleic acids) into eukariotic and prokariotic cells. Presumably the high voltage induces the formation of transient pores at lipidic-protein junctions, allowing introduction of exogenous macromolecules into the cells. In the genus Yersinia transformation by electroporation technique was applied to species like Y. enterocolitica, Y. pestis, Y. pseudotuberculosis (Conchas and Carniel, 1990) and Y. ruckeri (Cutrín et al., 1994) One consideration must be take into account when electroporation is attempted: all the buffers must be low ionic strengh solutions because the conductivity of the sample can result in arcing in the sample chamber. Usually this conflict is avoided when bacteria is highly concentrated on the lowest ionic buffers so that they can survive (Trevors J T, 1991). The protocol presented here, and the buffers used, are optimized for Y. ruckeri strains.
KeywordsCompetent Cell Electric Pulse High Ionic Strength Efficient Transformation System Competent Bacterium
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