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Electrotransformation of Yersinia ruckeri

  • Juan M. Cutrín
  • Alicia E. Toranzo
  • Juan L. Barja
Chapter
Part of the Springer Lab Manuals book series (SLM)

Abstract

Electroporation may be used for transferring macromolecules (proteins and nucleic acids) into eukariotic and prokariotic cells. Presumably the high voltage induces the formation of transient pores at lipidic-protein junctions, allowing introduction of exogenous macromolecules into the cells. In the genus Yersinia transformation by electroporation technique was applied to species like Y. enterocolitica, Y. pestis, Y. pseudotuberculosis (Conchas and Carniel, 1990) and Y. ruckeri (Cutrín et al., 1994) One consideration must be take into account when electroporation is attempted: all the buffers must be low ionic strengh solutions because the conductivity of the sample can result in arcing in the sample chamber. Usually this conflict is avoided when bacteria is highly concentrated on the lowest ionic buffers so that they can survive (Trevors J T, 1991). The protocol presented here, and the buffers used, are optimized for Y. ruckeri strains.

Keywords

Competent Cell Electric Pulse High Ionic Strength Efficient Transformation System Competent Bacterium 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag Berlin Heidelberg 2000

Authors and Affiliations

  • Juan M. Cutrín
    • 1
  • Alicia E. Toranzo
    • 1
  • Juan L. Barja
    • 1
  1. 1.Departamento de Mirobiología y Parasitología, Facultad de Biología, e Instituto de AcuiculturaUniversidad de Santiago de CompostelaSantiago de CompostelaSpain

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