Abstract
Bacillus amyloliquefaciens is a widely used host for production of industrial enzymes (Aunstrup et al. 1979). However, the species does not become physiologically competent for DNA transformation, unlike the related species B. subtilis. Therefore, alternative methods for introduction of plasmids into B. amyloliquefaciens have been developed. These include the protoplast transformation (Vehmaanperä 1988) and the electroporation methods (Vehmaanperä 1989). The latter is described below.
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References
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© 2000 Springer-Verlag Berlin Heidelberg
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Vehmaanperä, J. (2000). Bacillus amyloliquefaciens — Production Host for Industrial Enzymes. In: Eynard, N., Teissié, J. (eds) Electrotransformation of Bacteria. Springer Lab Manuals. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-04305-9_14
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DOI: https://doi.org/10.1007/978-3-662-04305-9_14
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-08593-2
Online ISBN: 978-3-662-04305-9
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