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General Principles of Bacteria Electrotransformation: Key Steps

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Electrotransformation of Bacteria

Part of the book series: Springer Lab Manuals ((SLM))

Abstract

Transfer of foreign information in the genome of cells is a key problem in cell biology and biotechnology. Bioelectrochemistry provided a major innovation when in 1982 Neumann introduced the electrotransformation method (Neumann, et al., 1982). The methodology is simple:

  1. 1.

    cells and plasmids are mixed

  2. 2.

    an electric field of high intensity is applied

  3. 3.

    the mixture is incubated to get the expression

  4. 4.

    a selection assay gives the transformants.

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Authors and Affiliations

  1. IPBS CNRS (UPR 9062), 118 Route de Narbonne, Toulouse, 31062, France

    Natalie Eynard & Justin Teissié

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  1. Natalie Eynard
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  2. Justin Teissié
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  1. Institut de Pharmacologie et Biologie Structurale, CNRS UPR 9062, 118 route de Narbonne, 31062, Toulouse cédex, France

    Natalie Eynard PhD  & Justin Teissié (Dr., Directeur de Recherces au CNRS) &  (Dr., Directeur de Recherces au CNRS)

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Eynard, N., Teissié, J. (2000). General Principles of Bacteria Electrotransformation: Key Steps. In: Eynard, N., Teissié, J. (eds) Electrotransformation of Bacteria. Springer Lab Manuals. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-04305-9_1

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  • DOI: https://doi.org/10.1007/978-3-662-04305-9_1

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