Detection and Purification of Rare Responders by Fixed-Time Flow Cytometry
Flow cytometrical analysis of cells stained with the calcium-sensitive dye indo-1 (Haugland, 1992) is now widely used for the measurement of cytosolic calcium [Ca2+]i of cells in response to external signals (Tárnok, 1996; 1997; Ulrich et al., 1996). These changes occur typically after stimulation of membrane bound receptors leading to an opening of intracellular calcium stores (Jin et al., 1994) and/or to a change of the activity of calcium channels located in the plasma membrane (Ulrich et al., 1996). These changes in [Ca2+]i are often transient, lasting in the second to minute time range.
KeywordsSurfactant DMSO Serotonin Arginine Pyruvate
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