Abstract
The term proteome is defined as: The entire protein complement expressed by a genome,a cell or tissue type (Wasinger et al. 1995, Wilkins et al. 1996). It follows that proteomics is the study of proteomes, however the terminology now encompasses a broader field of research and has branched into two specific disciplines; classical and functional proteomics.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Blackstock WP, Weir MP (1999) Proteomics: quantitative and physical mapping of cellular proteins. Trends Biotechnol 17: 121–127
Jensen ON, Podtelejnikov A, Mann M (1996) Delayed extraction improves specificity in database searches by matrix-assisted laser desorption/ionisation peptide maps. Rapid Commun Mass Spectrom 10: 1371–1378
Jensen ON, Mortensen P, Vorm O, Mann M (1997) Automation of matrix-assisted laser desorption/ionisation mass spectrometry using fuzzy logic feedback control. Anal Chem 69: 1706–1714
Kuster B, Mortensen P, Mann M (2000) Mass spectrometry allows direct identification of proteins in large genomes. Science Submitted
Mann M, Wilm M (1994) Error-tolerant identification of peptides in sequence databases by peptide sequence tags. Anal Chem 66: 4390–4399
Mercurio F, Zhu H, Murray BW, Shevchenko A, Bennett BL, Li J, Young DB, Barbosa M, Mann M, Manning A, Rao A (1997) IKK-1 and IKK-2: cytokine-activated IKB kinases essential for NF-KB activation. Science 278: 860–866
Morris HR, Paxton T, Dell A, Langhorne J, Berg M, Bordoli RS, Hoyes J, Bateman RH (1996) High-sensitivity collisionally-activated decomposition tandem mass spectrometry on a novel quadrupole/orthogonal-acceleration time-of-flight mass spectrometer. Rapid Commun Mass Spectrom 10: 889–896
Muzio M, Chinnaiyan AM, Kischkel FC, O’Rourke K, Shevchenko A, Ni J, Scaffidi C, Bretz JD, Zhang M, Gentz R, Mann M, Krammer PH, Peter ME, Dixit VM (1996) FLICE, a novel FADD-homologous ICE/CED-3-like protease, is recruited to the CD95 (Fas/APO-1) death-inducing signalling complex. Cell 85: 817–827
Neubauer G, King A, Rappsilber J, Calvio C, Watson M, Ajuh P, Sleeman J, Lamond A, Mann M (1998) Mass spectrometry and EST-database searching allows characterisation of the multi-protein spliceosome complex. Nat Genet 20: 46–50
Shevchenko A, Chernushevich I, Ens W, Standing KG, Thomson B, Wilm M, Mann M (1997) Rapid `de novo’ peptide sequencing by a combination of nanoelectrospray, isotopic labelling and a quadrupole/time-of-flight mass spectrometer. Rapid Commun Mass Spectrom 11: 1015–1024
Wasinger VC, Cordwell SJ, Cerpa-Poljak A, Yan JX, Gooley AA, Wilkins MR, Duncan MW, Harris R, Williams KL, Humphery-Smith I (1995) Progress with gene-product mapping of the Mollicutes: Mycoplasma genitalium. Electrophoresis 16: 1090–1094
Wilkins MR, Pasquali C, Appel RD, Ou K, Golaz O, Sanchez JC, Yan JX, Gooley AA, Hughes G, Humphery-Smith I, Williams KL, Hochstrasser DF (1996) From proteins to proteomes: large scale protein identification by two-dimensional electrophoresis and amino acid analysis. Bio/Technology 14: 61–65
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2000 Springer-Verlag Berlin Heidelberg
About this paper
Cite this paper
Bennett, K.L., Mørtz, E. (2000). The Wonderful World of Proteomics. In: Jégou, B., Pineau, C., Saez, J. (eds) Testis, Epididymis and Technologies in the Year 2000. Ernst Schering Research Foundation Workshop, vol 6. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-04050-8_3
Download citation
DOI: https://doi.org/10.1007/978-3-662-04050-8_3
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-662-04052-2
Online ISBN: 978-3-662-04050-8
eBook Packages: Springer Book Archive