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Immunoprecipitation

  • Paola Migliorini
Part of the Springer Lab Manual book series (SLM)

Abstract

Immunoprecipitation is a technique that allows the identification and separation of many antigens using antibodies that bind specifically to them. This procedure is based on the separation of the antibody-bound antigen from unbound antigens by means of reagents specific for the Fc portion of the immunoglobulins, usually protein A or protein G. Different preparations of unsolubilised protein A or G are commercially available. The matrix they are coupled to is usually sepharose, agarose or acrylic resins.

Keywords

Insoluble Protein Preimmune Serum Cold Lysis Buffer Sodium Bicarbonate Buffer Film Cassette 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag Berlin Heidelberg 1999

Authors and Affiliations

  • Paola Migliorini
    • 1
  1. 1.Department of Internal Medicine, Clinical Immunology UnitUniversity of PisaPisaItaly

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