Immunoprecipitation is a technique that allows the identification and separation of many antigens using antibodies that bind specifically to them. This procedure is based on the separation of the antibody-bound antigen from unbound antigens by means of reagents specific for the Fc portion of the immunoglobulins, usually protein A or protein G. Different preparations of unsolubilised protein A or G are commercially available. The matrix they are coupled to is usually sepharose, agarose or acrylic resins.
KeywordsInsoluble Protein Preimmune Serum Cold Lysis Buffer Sodium Bicarbonate Buffer Film Cassette
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