SEC Method Development

  • Sadao Mori
  • Howard G. Barth
Chapter
Part of the Springer Laboratory book series (SPLABORATORY)

Abstract

Polymer samples must be dissolved in the solvent used as the mobile phase. In non-aqueous SEC, solvents that can be used as the mobile phase are limited to those compatible with the stationary phase as well as those that are solvents for the polymer samples. However in special cases, samples can be dissolved in a different solvent from the mobile phase. For example, urea-formaldehyde resin dissolves in both N,N-dimethylformamide (DMF) and dimethylsulfoxide (DMSO), but DMSO is a much better solvent for the resin than DMF. Therefore, the resin is dissolved in DMSO and the solution injected into a system of polystyrene (PS) gel-stationary phase/DMF mobile phase. The other example is the SEC of isotactic polypropylene (iPP) with cyclohexane as the mobile phase at 70 °C. The sample iPP is insoluble in cyclohexane and is therefore dissolved in decalin at 140 °C and then diluted with hot cyclohexane and kept at about 70 °C prior to injection into the SEC system.

Keywords

Toluene Dextran Acrylate Dioxane Phthalate 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Rudin A (1971) J Polym Sci Part A-1 9: 2587CrossRefGoogle Scholar
  2. 2.
    Bleha T, Bakos D, Berek D (1977) Polymer 18: 897CrossRefGoogle Scholar
  3. 3.
    Mori S (1976) J Appl Polym Sci 20: 2157CrossRefGoogle Scholar
  4. 4.
    DIN 55672–1 Gelpermeationschromatographie (GPC) Teil 1: Tetrahydrofuran (THF) as Elutionsmittel (1995–02)Google Scholar
  5. 5.
    Matsuzaki T, Inouc Y, Ookubo T, Mori S (1980) J Liq Chromatogr 3: 353CrossRefGoogle Scholar
  6. 6.
    Mori S (1977) J Appl Polym Sci 21: 1921CrossRefGoogle Scholar
  7. 7.
    Mori S et al. (1996) Bunseki Kagaku (Analytical Chemistry in Japan) 45: 95CrossRefGoogle Scholar
  8. 8.
    Mori S, Suzuxt T (1980) Anal Chem 52: 1625CrossRefGoogle Scholar
  9. 9.
    Mori S, Suzuxt M (1984) J Liq Chromatogr 7: 1841CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1999

Authors and Affiliations

  • Sadao Mori
    • 1
  • Howard G. Barth
    • 2
  1. 1.Mie UniversityNagoyaJapan
  2. 2.Central Research and DevelopmentDuPont CompanyWilmingtonUSA

Personalised recommendations