Abstract
The determination of molecular weight (MW) averages and molecular weight distributions (MWD) of water-soluble polymers, such as nonionic and ionic synthetic polymers, proteins and peptides, by aqueous SEC sometimes encounters difficulties because of non-size exclusion effects. SEC is a separation technique based on molecular size and any polymers eluted at the same retention volume should have the same molecular size making it possible to calculate the MW or MWD of a polymer using a calibration curve constructed with polymer standards that are different from the polymer under investigation. Moreover, it can be assumed that species that elute earlier from SEC columns have higher MWs than those of the same type of polymer eluted later.
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References
Barth HG (1987) In: Provder T (ed) ACS Symposium Series No. 352, ACS, Washington, DC, chap 2
Barth HG, Regnier FE (1980) J Chromatogr 192: 275
Mori S (1989) Anal Chem 61: 530
Mori S, Kato M (1987) J Liq Chromatogr 10: 3113
Dubin PL, Prinsipi JM (1989) J Chromatogr 479: 159
Maezawa S, Takagi T (1983) J Chromatogr 280: 124
Link GW Jr, Keller PL, Stout RW, Banes AJ (1985) J Chromatogr 331: 253
Kato T, Tokuya T, Nozaki T, Takahashi A (1984) Polymer 25: 218
Gallec G, Anderson AW, Tsao GT (1984) J Polym Sci Polym Chem Ed 22: 287
Muller G, Yonnet C (1984) Makromol Chem Rapid Commun 5: 197
Wonnacott DM, Patton EV (1987) J Chromatogr 389: 103
Patton EV, Wonnacott DM (1987) J Chromatogr 389: 115
Domard A, Rinaudo M (1984) Polym Commun 25: 55
Kühn A, Förster S, Lösch R, Rommelfanger M, Rosenauer C, Schmidt M (1993) Makromol Chem Rapid Commun 14: 433
Yang YB, Verzele M (1987) J Chromatogr 391: 383
Richter WO, Schwandt P (1984) J Chromatogr 288: 212
Irvine GB (1987) J Chromatogr 404: 215
Mascher E, Lundahl P (1989) J Chromatogr 476: 147
Mori S, unpublished data
Dimenna GP, Segall HJ (1981) J Liq Chromatogr 4: 639
Ahmed F, Modrek B (1992) J Chromatogr 599: 25
Visser S, Slangen CJ, Robben Ajpm (1992) J Chromatogr 599: 205
Cauret N, CÔTÉ J, Archambault J, André G (1992) J Chromatogr 594: 179
Himmel ME, Perna PJ, Mcdonell MW (1982) J Chromatogr 240: 155
Ellegren H, Laas T (1989) J Chromatogr 467: 217
Van Dijk Japp, Varkevisser FA, Smit JAM (1987) J Polym Sci Part B Polym Phys 25: 149
Stone RG, Krasowski JA (1981) Anal Chem 53: 736
Eremeeva TE, Bykova TO (1993) J Chromatogr 639: 159
Praznik W, Beck Rhf, Eigner WD (1987) J Chromatogr 387: 467
Devries JX (1989) J Chromatogr 465: 297
Lambert F, Milas M, Rinauno M (1982) Polym Bull 7: 185
Pellinen J, Salkinoja-Salonen M (1985) J Chromatogr 322: 129
Motohashi N, Nakamichi Y, Mori I, Nishikawa H, Umemoto J (1988) J Chromatogr 435: 335
Shimada E, Matsumura G (1992) J Chromatogr 627: 43
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© 1999 Springer-Verlag Berlin Heidelberg
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Mori, S., Barth, H.G. (1999). Aqueous Size Exclusion Chromatography. In: Size Exclusion Chromatography. Springer Laboratory. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-03910-6_11
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DOI: https://doi.org/10.1007/978-3-662-03910-6_11
Publisher Name: Springer, Berlin, Heidelberg
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