Production of Pea Seed Lipoxygenases in Escherichia coli

  • R. K. Hughes
  • Z. Wu
  • D. S. Robinson
  • R. Casey
Conference paper

Summary

The two major forms of lipoxygenase from pea seeds (LOX 2 and 3) have been cloned and expressed as soluble, active, non-fusion proteins in Escherichia coli. Unambiguous measurements to date comparing the homogeneously purified LOX 3 from E. coli and pea seeds have confirmed the authenticity of this recombinant product. Preliminary comparisons of LOX 2 in partiallγ-purified extracts suggested that the recombinant product was also authentic. Comparison of the enzymic properties of the two isoforms has indicated that they are quite different.

Keywords

Peroxide HPLC Hexane Aldehyde Carbonyl 

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Copyright information

© Springer-Verlag Berlin Heidelberg 1998

Authors and Affiliations

  • R. K. Hughes
    • 1
  • Z. Wu
    • 2
  • D. S. Robinson
    • 2
  • R. Casey
    • 1
  1. 1.Department of Applied GeneticsJohn Innes CentreNorwichUK
  2. 2.Procter Department of Food ScienceThe University of LeedsLeedsUK

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