Production of Pea Seed Lipoxygenases in Escherichia coli
The two major forms of lipoxygenase from pea seeds (LOX 2 and 3) have been cloned and expressed as soluble, active, non-fusion proteins in Escherichia coli. Unambiguous measurements to date comparing the homogeneously purified LOX 3 from E. coli and pea seeds have confirmed the authenticity of this recombinant product. Preliminary comparisons of LOX 2 in partiallγ-purified extracts suggested that the recombinant product was also authentic. Comparison of the enzymic properties of the two isoforms has indicated that they are quite different.
KeywordsPeroxide HPLC Hexane Aldehyde Carbonyl
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