Summary
This report describes the manipulation of the protein quality of potato tubers by genetic engineering. The content of the sulfur-containing amino acid methionine, an essential amino acid which is limiting in potato protein, was increased by expression of heterologous genes encoding proteins rich in methionine.
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CSAPO J., CSAPO-KISS Z., FOLESTAD S., TIVESTEN A., 1994. Mercaptoethanesulphonic acid as a protecting and hydrolysing agent for the determination of the amino acid composition of proteins using an elevated temperature for protein hydrolysis. Anal. Chem. Acta. 289, 105–111.
HULME J.S., HIGGINS E.S., SHIELDS R., 1992. An efficient genotype-independent method for regeneration of potato plants from leaf tissue. Plant Cell Tiss. Org. Cult. 31, 161–167.
JEFFERSON R., GOLDSBROUGH A., BEVAN M.W., 1990. Transcriptional regulation of a patatin-1 gene in potato. Plant Mol. Biol. 14, 995–1006.
MANIATIS T., FRITSCH E.F., SAMBROOK J., 1982. Molecular Cloning: a Laboratory Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
SUN S.S.M., LARKINS B.A., 1993. Transgenic plants for improving seed storage proteins. In: Kung, S.D. and Wu, R. (Ed.): Transgenic Plants. Academic Press, San Diego, Ca, p. 339–372.
TU H.M., GODFREY L.W., SUN S.M., 1994. Expression of the Brazil nut methionine- rich protein in transgenic potato plants. In: Belknap, W.R., Vayda, M.E. and Park, W.D. (Ed.): The Molecular and Cellular Biology of the Potato. CAB International, Wallingford, UK., p. 209–220.
YANG M.S., ESPINOZA N.O., NAGPALA P.G., 1989. Expression of a synthetic gene for improved protein quality in transformed potato plants. Plant Sci. 64, 99–111.
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© 1998 Springer-Verlag Berlin Heidelberg
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Randhawa, G., Lyon, J., Harris, N., Davies, H.V., Machray, G.C. (1998). Manipulation of Potato Tuber Protein Quality through Genetic Engineering. In: Guéguen, J., Popineau, Y. (eds) Plant Proteins from European Crops. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-03720-1_12
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DOI: https://doi.org/10.1007/978-3-662-03720-1_12
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-662-03722-5
Online ISBN: 978-3-662-03720-1
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