Summary
Wheat and tritordeum plants transformed with HMW glutenin 1Ax1 and 1Dx5 genes have been obtained with high efficiency. In both species, most of the lines analyzed so far show expression levels of HMW glutenin transgenes higher than or comparable to those of the native gene. Transgene copy number does not appear to be correlated with the level of expression. Dough functionality analyses (mixograph) are in progress to determine the effects of these subunits on breadmaking properties.
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ANDERSON O.D., GREENE F.C., YIP RE., HALFORD N.G., SHEWRY P.R., MALPICA-ROMERO J.M., 1989. Nucleotide sequences of the two high-molecular-weight glutenin genes from the D-genome of a hexaploid bread wheat, Triticum aestivum L. cv. Cheyenne. Nucleic Acid Research 17, 461–462.
BARCELO P., LAZZERI P.A., 1995. Transformation of cereals by microprojectile bombardment of immature inflorescence and scutellum tissues. In: Jones, H. (ed.): Methods in Molecular Biology: Plant Gene Transfer and Expression Protocols Humana Press Inc., Totowa, NJ, 49, 113–123.
Christensen A.H., Quail P.H., 1996. Ubiquitin promoter-based vectors for high-level expression of selectable and/or screenable marker genes in monocotyledonous plants. Transgenic Research 5, 213–218.
HALFORD N.G., FIELD J.M., BLAIR H., URWIN P., MOORE K., ROBERT L., THOMPSON R., FLAVELL R.B., TATHAM A.S., SHEWRY P.R., 1992. Analysis of HMW glutenin subunits encoded by chromosome 1A of bread wheat (Triticum aestivum L.) indicates quantitative effects on grain quality. Theoretical and Applied Genetics 83, 373–378.
MARTIN A., SANCHEZ-MONGE E., 1982. Cytology and morphology of the amphiploid Hordeum chilense x Triticum turgidum conv. durum. Euphytica,3l, 261–267.
MCELROY O., ZHANG W, CA J., WU R., 1990. Isolation of and efficient Actin promoter for use in rice transformation. Plant Cell 2, 163–171.
PAYNE P.I., 1987. Genetics of wheat storage proteins and the effect of allelic variation on breadmaking quality. Annual Review of Plant Physiology,38, 141–153.
SHEWRY P.R., HALFORD N.G., TATHAM A.S., 1992. The high molecular weight subunits of wheat glutenin. Journal of Cereal Science 15, 105–120.
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© 1998 Springer-Verlag Berlin Heidelberg
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Barro, F. et al. (1998). Expression of HMW Glutenin Genes in Transgenic Wheat and Tritordeum Plants. In: Guéguen, J., Popineau, Y. (eds) Plant Proteins from European Crops. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-03720-1_11
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DOI: https://doi.org/10.1007/978-3-662-03720-1_11
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