Skip to main content

Experimental In Vitro Approaches to the Study of Mouse Primordial Germ Cell Development

  • Conference paper
Testicular Function: From Gene Expression to Genetic Manipulation

Part of the book series: Ernst Schering Research Foundation Workshop ((3368,volume 3))

Abstract

Primordial germ cells (PGCs) are the founder cells of germ line. In all mammalian species studied, including humans, they originate extragonadally during early embryogenesis before moving toward and colonizing the gonadal ridges. Upon entering the gonads, PGCs differentiate into meiotic oocytes in the fetal ovary and prospermatogonia in the fetal testis.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 39.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 54.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  • Buehr M (1997) The primordial germ cells of mammals: some current perspectives. Exp Cell Res 232:194–207

    Article  PubMed  CAS  Google Scholar 

  • Byskov AG, Saxen L (1976) Induction of meiosis in fetal mouse testis in vitro. Dey Biol 52:193–200

    Article  CAS  Google Scholar 

  • Cheng L, Gearing DP, White LS, Compton DL, Schooley K, Donovan P (1994) Role of leukemia inhibitory factor and its receptor in mouse primordial germ cell growth. Development 120:3145–3153

    PubMed  CAS  Google Scholar 

  • Cooke JE, Heasman J, Wylie CC (1996) The role of interleukin-4 in the regulation of mouse primordial germ cell numbers. Dev Biol 174:14–21

    Article  PubMed  CAS  Google Scholar 

  • De Felici M, Dolci S (1989) In vitro adhesion of mouse fetal germ cells to extracellular matrix components. Cell Differ Dev 26:87–96

    Article  PubMed  Google Scholar 

  • De Felici M, Dolci S (1991) Leukemia inhibitory factor sustains the survival of mouse primordial germ cells cultured on TM4 feeder layers. Dev Biol 147:281–284

    Article  PubMed  Google Scholar 

  • De Felici M, McLaren A (1982) Isolation of mouse primordial germ cells. Exp Cell Res 142:476–482

    Article  PubMed  Google Scholar 

  • De Felici M, McLaren A (1983) In vitro culture of mouse primordial germ cells. Exp Cell Res 144:417–427

    Article  PubMed  Google Scholar 

  • De Felici M, Pesce M (1994) Growth factors in mouse primordial germ cell migration and proliferation. Progr Growth Factor Res 5:135–145

    Article  Google Scholar 

  • De Felici M, Pesce M (1995) Immunoaffinity purification of migratory mouse primordial germ cells. Exp Cell Res 216:277–279

    Article  PubMed  Google Scholar 

  • De Felici M, Dolci S, Pesce M (1992) Cellular and molecular aspects of mouse primordial germ cell migration and proliferation in culture. Int J Dev Biol 36:205–213

    PubMed  Google Scholar 

  • De Felici M, Dolci S, Pesce M (1993) Proliferation of mouse primordial germ cells in vitro: a key role for cAMP. Dev Biol 157:227–280

    Article  Google Scholar 

  • De Felici M (1997) Isolation and culture of germ cells from mouse embryo. In: Celis JC (ed) Cell biology: a laboratory handbook. Academic, New York, pp 68–80

    Google Scholar 

  • Dolci S, De Felici M (1990) A study of meiosis in chimeric mouse fetal gonads. Development 109:37–40

    PubMed  CAS  Google Scholar 

  • Dolci S, Williams DE, Ernst MK, Resnick JL, Braman CI, Fock LF, Lyman SD, Boswell SH, Donovan PJ (1991) Requirements for mast cell growth factor for primordial germ cell survival in culture. Nature 352:809–811

    Article  PubMed  CAS  Google Scholar 

  • Dolci S, Pesce M, De Felici M (1993) Combined action of stem cell factor, leukemia inhibitory factor, and cAMP on in vitro proliferation of mouse primordial germ cells. Mol Repr Dev 35:134–139

    Article  CAS  Google Scholar 

  • Donovan PJ (1994) Growth factor regulation of mouse primordial germ cell development. In: Pedersen RA (ed) Current topics in developmental biology. Academic, New York, pp 189–225

    Google Scholar 

  • ffrench-Constant C, Hollingsworth A, Heasman J, Wylie CC (1991) Response to fibronectin of mouse primordial germ cells before, during and after migration. Development 113:1365–1373

    PubMed  CAS  Google Scholar 

  • Garcia-Castro M, Anderson R, Heasman J, Wylie CC (1997) Interactions between germ cells and extracellular matrix glycoproteins during migration and gonad assembly in the mouse embryo. J Cell Biol 138:471–480

    Article  PubMed  CAS  Google Scholar 

  • Godin I, Wylie CC (1991) TGFß 1 inhibits proliferation and has a chemotropic effect on mouse primordial germ cells in culture. Development 113:1451–1457

    PubMed  CAS  Google Scholar 

  • Godin I, Deed R, Cooke J, Zsebo K, Dexter M, Wylie CC (1991) Effect of the Steel gene product on mouse primordial germ cells in culture. Nature 352:807–809

    Article  PubMed  CAS  Google Scholar 

  • Kawase E, Yamamoto HKH, Nakatsui N (1994) Tumor necrosis factor α (TNF-α) stimulates proliferation of mouse primordial germ cells in culture. Dev Biol 162:91–95

    Article  Google Scholar 

  • Koshimizu U, Watanabe M, Nakatsuji N (1995) Retinoic acid is potent growth activator of mouse primordial germ cell in vitro. Dev Biol 168:683–685

    Article  PubMed  CAS  Google Scholar 

  • Koshimizu U, Taga T, Watanabe M, Saito M, Shirayoshi Y, Kishimoto T, Nakatsuji N (1996) Functional requirement of gp 130-mediated signaling for growth and survival of mouse germ cells in vitro and derivation of embryonic germ (EG) cells. Development 122:1235–1242

    PubMed  CAS  Google Scholar 

  • Mastubara N, Takahashi Y, Nishina Y, Mukouyama Y, Yanagisawa M, Watanabe T, Nakano T, Nomura K, Arita H, Nishimune Y, Obinata Y, Matsui Y (1996) A receptor proteinchinasy, Sky, and its ligand Gas 6 are expressed in gonads and support primordial germ cell growth or survival in culture. Dev Biol 180:499–510

    Article  Google Scholar 

  • Matsui Y, Zsebo K, Hogan BLM (1990) Embryonic expression of a haemotopoietic growth factor encoded by the Steel locus and the ligand of c-kit. Nature 347:666–669

    Article  Google Scholar 

  • Matsui Y, Toksoz D, Nishikawa S, Nishikawa S-I, Williams D, Zsebo K, Hogan BLM (1991) Effect of Steel factor and leukemia inhibitory factor on murine primordial germ cells in culture. Nature 353:750–752

    Article  PubMed  CAS  Google Scholar 

  • Matsui Y, Zsebo K, Hogan BLM (1992) Derivation of pluripotential embryonic stem cells from murine primordial germ cells in culture. Cell 70:841–847

    Article  PubMed  CAS  Google Scholar 

  • McLaren A (1994) Germline and soma: interactions during early mouse development. Semin Dev Biol 5:43–49

    Article  Google Scholar 

  • McLaren A, Southee D (1997) Entry of mouse embryonic germ cells into meiosis. Dev Biol 187:107–113

    Article  PubMed  CAS  Google Scholar 

  • Pesce M, Farace MG, Piacentini M, Dolci S, De Felici M (1993) Stem cell factor and leukemia inhibitory factor promote primordial germ cell survival by suppressing programmed cell death (apoptosis). Development 118:1089–1094

    PubMed  CAS  Google Scholar 

  • Pesce M, De Felici M (1994) Apoptosis in mouse primordial germ cells: a study by transmission and scanning electron microscope. Anat Embryol 189:435–440

    Article  PubMed  CAS  Google Scholar 

  • Pesce M, De Felici M (1995) Purification of mouse primordial germ cells by Mini-MACS magnetic separation system. Dev Biol 170:722–72

    Article  PubMed  CAS  Google Scholar 

  • Pesce, M, Canipari R, Ferri GL, Siracusa G, De Felici M (1996a) Pituitary adenylate cyclase activating-polypeptide (PACAP) stimulates adenylate cyclase and promotes proliferation of mouse primordial germ cells. Development 122:215–221

    PubMed  CAS  Google Scholar 

  • Pesce M, Di Carlo A, Cerrito MG, De Felici M (1996b) All-trans retinoic acid effects on mouse primordial germ cells in culture. Biol Repr 54:414

    Google Scholar 

  • Pesce M, Di Carlo A, De Felici M (1997) The c-kit receptor is involved in the adhesion of mouse primordial germ cells to somatic cells in culture. Mech Dev (in press)

    Google Scholar 

  • Rich IV (1995) Primordial germ cells are capable of producing cells of the hematopoietic system in vitro. Blood 86:463–472

    PubMed  CAS  Google Scholar 

  • Resnick JL, Bixler LS, Cheng L, Donovan PJ (1992) Long term proliferation of mouse primordial germ cells in culture. Nature 359:550–551

    Article  PubMed  CAS  Google Scholar 

  • Stewart CL, Kaspar P, Brunet LJ, Bhatt H, Gadi I, Kontgen F, Abbondanzo SJ (1992) Blastocyst implantation depends on maternal expression of leukemia inhibitory factor. Nature 359:76–79

    Article  PubMed  CAS  Google Scholar 

  • Stewart CL, Gadi I, Bhatt H (1994) Stem cell from primordial germ cells reenter the germ line. Dev Biol 161:626–628

    Article  PubMed  CAS  Google Scholar 

  • Tam PPL, Snow MHL (1981) Proliferation and migration of primordial germ cells during compensatory growth in mouse embryo. J Embryol Exp Morphol 64:133–147

    PubMed  CAS  Google Scholar 

  • Ware C, Horowitz MC, Renshaw BR, Hunt JS, Liggitt D, Koblar SA, Gliniak BC, McKenna HJ, Papayannopoulos T, Thoma B, Cheng L, Donovan PJ, Peschon J, Bartlett PF, Willis CR, Wright BD, Carpenter MK, Davison BL, Gearing DP (1995) Targeted disruption of the low-affinity leukemia inhibitory factor receptor gene causes placental, skeletal, neural and metabolic defects and results in perinatal death. Development 121:1283–1299

    PubMed  CAS  Google Scholar 

  • Wylie CC, Heasman J (1993) Migration, proliferation and potency of primordial germ cells. Seminars in Dev Biol 4:161–170

    Article  Google Scholar 

  • Wylie CC, Stott D, Donovan PJ (1986) Primordial germ cell emigration. In: Browder L (ed) The cellular basis of morphogenesis. Plenum, New York, pp 433–450

    Chapter  Google Scholar 

Download references

Authors

Editor information

M. Stefanini C. Boitani M. Galdieri R. Geremia F. Palombi

Rights and permissions

Reprints and permissions

Copyright information

© 1998 Springer-Verlag Berlin Heidelberg

About this paper

Cite this paper

De Felici, M., Di Carlo, A., Dolci, S., Pesce, M. (1998). Experimental In Vitro Approaches to the Study of Mouse Primordial Germ Cell Development. In: Stefanini, M., Boitani, C., Galdieri, M., Geremia, R., Palombi, F. (eds) Testicular Function: From Gene Expression to Genetic Manipulation. Ernst Schering Research Foundation Workshop, vol 3. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-03671-6_2

Download citation

  • DOI: https://doi.org/10.1007/978-3-662-03671-6_2

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-662-03673-0

  • Online ISBN: 978-3-662-03671-6

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics