Abstract
Countercurrent chromatography (CCC) is a separation technique which relies on the partition of a sample between two immiscible solvents, the relative proportions of solute passing into each of the two phases being determined by the respective partition coefficients. It is an all-liquid method which is characterized by the absence of a solid support, and thus has the following advantages over other chromatographic techniques:
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no irreversible adsorption of the sample (solutes are readily retained by a solid support; the adsorption effects are often manifested by elution curves which show tailing of the substances under consideration)
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quantitative recovery of the introduced sample
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risk of sample denaturation greatly reduced
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ow solvent consumption
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favourable economics.
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Hostettmann, K., Marston, A., Hostettmann, M. (1998). Countercurrent Chromatography. In: Preparative Chromatography Techniques. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-03631-0_7
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