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Genetic Diversity of Ralstonia solanacearum in Japan

  • K. Tsuchiya
  • M. Horita
Chapter

Abstract

Five races and 5 biovars of Ralstonia solanacearum have been described and they differ in host range, pathogenicity and physiological properties. In Japan, R. solanacearum was isolated from various hosts, and 2 races and 4 biovars were identified. RFLP and PCR-RFLP analysis of the 16S and/or 23S ribosomal DNA region showed that the Japanese race 1/biovars 1 – 4 strains were similar to Asian biovars 3, 4 and 5. Japanese race 3/biovar 2 strains were very similar to strains of American biovar 1 and Blood disease bacteria (BDB) from banana. RFLP and rep-PCR analyses of Japanese strains differing in geographical origin and host resulted in two major clusters. Cluster 1 comprised Japanese race 1/biovars 1 – 4. Cluster 2 comprised Japanese race 3/biovar 2. Cluster 1 was further subdivided into 7 groups by RFLP analysis and 4 groups by rep-PCR analysis, respectively. Clusters correlated with a race, biovar, tobacco compatibility and original host.

Keywords

Bacterial Wilt Sweet Pepper Ralstonia Solanacearum Restriction Fragment Length Polymorphism Japanese Strain 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag Berlin Heidelberg 1998

Authors and Affiliations

  • K. Tsuchiya
  • M. Horita

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