Abstract
Ninety seven Philippine and 24 banana-infecting strains from other countries were hybridized with probes which harbor the genes for extracellular polysaccharides (E36, 07, Q50), endoglucanase (pHE3), tryptophan biosynthesis (pT161), a random clone (pZ1217) from the gene bank of a Philippine ginger strain and a cloned repetitive element from a Philippine banana strain. The same set was also analyzed by polymerase chain reaction (PCR) using REP, ERIC and BOX primers (rep-PCR). At 65% level of similarity, five and six clusters resulted in the composite restriction fragment length polymorphism (RFLP) and PCR analysis, respectively. Only the cloned repetitive element in the RFLP analysis and REP and BOX primers in PCR analysis detected polymorphism among the Philippine banana isolates. In all cases, more than 96% of the strains belonged to one haplotype and the rest to the other haplotypes. Based on the above analysis, the banana wilt (moko) and the fruit blackening (bugtok) strains from the Philippines were genetically indistinguishable.
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© 1998 Springer-Verlag Berlin Heidelberg
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Raymundo, A.K., Aves-ILagan, Y., Denny, T.P. (1998). Analysis of Genetic Variation of a Population of Banana Infecting Strains of Ralstonia solanacearum . In: Prior, P., Allen, C., Elphinstone, J. (eds) Bacterial Wilt Disease. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-03592-4_8
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DOI: https://doi.org/10.1007/978-3-662-03592-4_8
Publisher Name: Springer, Berlin, Heidelberg
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