Genetic Diversity of Ralstonia solanacearum Race 3 in Western Europe determined by AFLP, RC-PFGE and Rep-PCR
Recently, several outbreaks of brown rot caused by Ralstonia solanacearum (Smith) Smith biovar 2, race 3 were reported in Western Europe. Knowledge of the variation in the population of the target pathogen forms the basis of epidemiological studies and control measures. In this study, the genetic diversity of race 3 strains from France, The Netherlands and the UK was determined with different molecular techniques. AFLP and pulsed-field gel electrophoresis of genomic DNA digested by rare-cutting restriction enzymes (RC-PFGE) were able to separate race 3 strains in different groups, indicating that several clonal lines are present in Europe. ERIC- and BOX-PCR were less discriminative. No correlation was found between geographical origin and profiles obtained.
KeywordsAmplify Fragment Length Polymorphism Ralstonia Solanacearum Wild Solanum Species Solanacearum Strain Amplify Fragment Length Polymorphism Technique
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