The Development of a Biological Control Agent against Ralstonia solanacearum Race 3 in Kenya
Indigenous populations of R. solanacearum affecting potato in Kenya were extensively characterised by a multifaceted approach including genomic fingerprinting by macro-restriction of genomic DNA resolved by pulsed field gel electrophoresis. From these studies clear isolate types were observed with varying frequency. Representative of these isolate types were mutated to a non-pathogenic form by 1) insertion of a DNA omega element into the Hrp cluster, or 2) deletion mutation through the insertion and eviction of the sacB gene of Bacillus subtilus. Inoculation of potato plants with the non-pathogenic omega mutants conferred a reduction in disease symptoms in the order of 30%.
KeywordsSucrose Agar Electrophoresis Bacillus Pseudomonas
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