Abstract
Nitric oxide synthase (NOS) activity was detected in tobacco leaf tissues by arginine/citrulline assay procedure. A protein reactive to anti-NOS antibodies was also detected by western blot analysis. Both NOS activity and the NOS immunoreactive protein increased in tobacco leaves during the hypersensitive reaction against Ralstonia solanacearum. Infiltration of a NO-releasing compound into tobacco leaves caused HR-like symptoms. In contrast, infiltration of a NOS inhibitor delayed the onset of the HR. These results indicate that NOS activity is increased in the hypersensitive tissue and that NO may be involved in the HR induction.
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© 1998 Springer-Verlag Berlin Heidelberg
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Huang, JS., Knopp, J.A. (1998). Involvement of Nitric Oxide in Ralstonia solanacearum-Induced Hypersensitive Reaction in Tobacco. In: Prior, P., Allen, C., Elphinstone, J. (eds) Bacterial Wilt Disease. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-03592-4_32
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DOI: https://doi.org/10.1007/978-3-662-03592-4_32
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