Studies on the Molecular Basis for Pathogenicity and Host Specificity in Strains of Ralstonia solanacearum Pathogenic to Banana

  • R. Thwaites
  • S. Eden-Green
  • J. Mansfield
  • S. Seal
Chapter

Abstract

The genetic variation among strains of Ralstonia solanacearum belonging to race 2 and related bacteria was investigated by polymerase chain reaction amplification with random primers. Cluster analysis of the resulting genetic fingerprints grouped the various isolates in accordance with previously reported findings. In addition to this investigation we are currently characterising a transposon-induced mutant R. solanacearum strain which has lost pathogenicity on its natural host, banana, while retaining the ability to wilt tomato under experimental conditions.

Keywords

Agarose Bromide Pseudomonas Ethidium Indonesia 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Ma QS, Chang MF, Tang JL, Feng JX, Fan MJ, Han B, Lui T (1988) Identification of DNA sequences involved in host specificity in the pathogenesis of Pseudomonas solanacearum strain T2005. Mol Plant-Microbe Interact 1: 169 – 174CrossRefGoogle Scholar
  2. Seal SE, Jackson LA, Daniels MJ (1992) Use of tRNA consensus primers to indicate subgroups of Pseudomonas solanacearum by polymerase chain reaction amplification. Appl Environ Microbiol 58: 3759 – 3761PubMedGoogle Scholar
  3. Seal SE, Jackson LA, Young JPW, Daniels MJ (1993) Differentiation of Pseudomonas solanacearum, Pseudomonas syzygii, Pseudomonas pickettii and the Blood Disease Bacterium by partial 16S rRNA sequencing: construction of oligonucleotide primers for sensitive detection by polymerase chain reaction. J Gen Microbiol 139:1587 – 1594PubMedCrossRefGoogle Scholar
  4. Taghavi M, Hayward C, Sly LI, Fegan M (1996) Analysis of the phylogenetic relationships of strains of Burkholderia solanacearum, Pseudomonas syzygii, and the blood disease bacterium of banana based on 16S rRNA gene sequences. Int J S y st Bacteriol 46: 10 – 15CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1998

Authors and Affiliations

  • R. Thwaites
  • S. Eden-Green
  • J. Mansfield
  • S. Seal

There are no affiliations available

Personalised recommendations