Studies on the Molecular Basis for Pathogenicity and Host Specificity in Strains of Ralstonia solanacearum Pathogenic to Banana
The genetic variation among strains of Ralstonia solanacearum belonging to race 2 and related bacteria was investigated by polymerase chain reaction amplification with random primers. Cluster analysis of the resulting genetic fingerprints grouped the various isolates in accordance with previously reported findings. In addition to this investigation we are currently characterising a transposon-induced mutant R. solanacearum strain which has lost pathogenicity on its natural host, banana, while retaining the ability to wilt tomato under experimental conditions.
KeywordsAgarose Bromide Pseudomonas Ethidium Indonesia
Unable to display preview. Download preview PDF.
- Seal SE, Jackson LA, Young JPW, Daniels MJ (1993) Differentiation of Pseudomonas solanacearum, Pseudomonas syzygii, Pseudomonas pickettii and the Blood Disease Bacterium by partial 16S rRNA sequencing: construction of oligonucleotide primers for sensitive detection by polymerase chain reaction. J Gen Microbiol 139:1587 – 1594PubMedCrossRefGoogle Scholar