Advertisement

Selection of Ralstonia solanacearum Race 3 Specific Monoclonal Antibodies from Combinatorial Libraries

  • R. A. Griep
  • C. van Twisk
  • J. M. van der Wolf
  • J. R. van Beckhoven
  • A. Schots
Chapter

Abstract

Recombinant antibodies against the lipopolysaccharide of Ralstonia solanacearum (biovar 2, race 3) were successfully selected by phage display from a large combinatorial antibody library within six weeks. The isolated monoclonal scFv antibodies reacted both in ELISA and in IF with race 3 strains, but with only few strains belonging to other races. They were more specific than the polyclonal antiserum which is routinely used for brown rot diagnosis and showed considerably fewer non-specific reactions with saprophytic bacteria. They may be used for brown rot diagnosis in Europe.

Keywords

Phage Display Ralstonia Solanacearum scFv Antibody Filamentous Phage Phage Antibody 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Alvarez AM, Benedict AA (1990) Monoclonal antibodies for the identification of plant pathogenic bacteria: potential applications to Ralstonia solanacearum. In: Middleton, KJ and Hayward AC (eds) Bacterial Wilt of Groundnut. Proceedings of an ACIAR/ICRISAT collaborative research planning meeting held at Genting Highlands, Malaysia 18 – 19 March 1990 ACIAR Proceedings 31:26 – 31 ACIAR, CanberraGoogle Scholar
  2. Elphinstone JG, Hennessy J, Wilson JK, Stead DK (1996) Sensitivity of different methods for the detection of Ralstonia solanacearum (Smith) Smith in potato tuber extract. OEPP/EPPO Bulletin 26 in pressGoogle Scholar
  3. Griffiths AD, Williams SC, Hartley O, Tomlinson IM, Waterhouse P, Crosby WL, Kontermann RE, Jones PT, Low NM, Allison TJ, Prospero TD, Hoogenboom HR, Nissim A, Cox JPL, Harrison JL, Zaccolo M, Gherardi E, Winter G (1994) Isolation of High affinity antibodies directly from large synthetic repertoires. EMBO J 13:3245 – 3260PubMedGoogle Scholar
  4. Hoogenboom HR, Griffiths AD, Johnson KS, Chisswell DJ, Hudson P, Winter G (1991) Multi-subunit proteins on the surface of filamentous phage: Methodologies for disP laY ing antibody (Fab) heavy and light chains. Nucleic Acids Res 19:4133 – 4137PubMedCrossRefGoogle Scholar
  5. He LY (1986) Bacterial wilt in the People’s Republic of China. In: Persley, G.J. (ed.) In: Bacterial Wilt Disease and the South Pacific. ACIAR Proceedings 13:40 – 8 ACIAR, Canberra. AustraliaGoogle Scholar
  6. Janse JD (1988) A detection method for Ralstonia solanacearum in symptomless potato tubers and some data on its sensitivity and specificity. OEPP/EPPO Bull 18:343 – 351CrossRefGoogle Scholar
  7. McCafferty J, Griffiths AD, Winter G, Chiswell D (1990) Phage antibodies: filamentous phage displaying antibody variable domains. Nature 348:552 – 554PubMedCrossRefGoogle Scholar
  8. Robinson-Smith A, Jones P, Elphinstone JG, Forde SMD (1995) Production of antibodies to Ralstonia solanacearum, the causative agent of bacterial wilt. Food and Agric Immunol 7:67 – 79CrossRefGoogle Scholar
  9. Vaughan TJ, Williams AJ, Pritchard K, Osbourn JK, Pope AR, Earnshaw JC, McCafferty J, Hodits RA, Wilton J, Johnson K (1996) Human antibodies with sub-nanomolar affinities isolated from a large non-immunized phage display library. Nature Biotech 14:309 – 314CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1998

Authors and Affiliations

  • R. A. Griep
  • C. van Twisk
  • J. M. van der Wolf
  • J. R. van Beckhoven
  • A. Schots

There are no affiliations available

Personalised recommendations