Selection of Ralstonia solanacearum Race 3 Specific Monoclonal Antibodies from Combinatorial Libraries
Recombinant antibodies against the lipopolysaccharide of Ralstonia solanacearum (biovar 2, race 3) were successfully selected by phage display from a large combinatorial antibody library within six weeks. The isolated monoclonal scFv antibodies reacted both in ELISA and in IF with race 3 strains, but with only few strains belonging to other races. They were more specific than the polyclonal antiserum which is routinely used for brown rot diagnosis and showed considerably fewer non-specific reactions with saprophytic bacteria. They may be used for brown rot diagnosis in Europe.
KeywordsPhage Display Ralstonia Solanacearum scFv Antibody Filamentous Phage Phage Antibody
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