Solid Phase Libraries of Glycopeptide Templates in the Study of Complex Oligosaccharide-Receptor Interactions
Carbohydrate binding proteins are involved in myriad biological functions including communication and adhesion between cells, adhesion of bacteria or viruses, activation of the innate immune system, leukocyte rolling, hepatic clearing of aged serum proteins, and sorting of newly synthesized glycoproteins (Dwek 1996; Varki 1993). Based on their mode of binding they have been divided into three major groups. The E-, L-, and P-selectins and the galectins are of the calcium-dependent C-type lectins, which bind their ligand mainly through coordination of two vicinal hydroxy groups of a single sugar moiety to a bound calcium ion in the carbohydrate recognition domain (CRD), and the surrounding sugars of the oligosaccharide ligand add to the binding specificity through relatively weak additional interactions. Due to the nature of this interaction the specificity of selectin binding is quite broad (Kretzsch-mar et al. 1997). However, the high in vivo activity observed with selectin binding has yet to be explained.
KeywordsPorcine Liver Carbohydrate Binding Protein Naphthoic Acid Protein Carbohydrate Interaction Carbohydrate Interaction Carbohydrate
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