A Water Channel Network in Cell Membranes of the Filter Chamber of Homopteran Insects

  • P. Bron
  • V. Lagrée
  • A. Froger
  • I. Pellerin
  • S. Deschamps
  • J.-F. Hubert
  • C. Delamarche
  • A. Cavalier
  • J.-P. Rolland
  • J. Gouranton
  • D. Thomas
Conference paper
Part of the Centre de Physique des Houches book series (LHWINTER, volume 10)

Abstract

Water is the most ubiquitous molecule in the living cell and movement of water across the cell membrane accompanies fundamental cell functions. All biological membranes exhibit some water permeability as a result of diffusion through the lipid bilayer and osmotic gradients constitute the driving force for water flow. Osmotic water permeability is therefore of the highest relevance. However, some cells have the ability to transport water across their cell membrane at greatly accelerated rates, for example mammalian red blood cells, epithelial cells of the renal proximal tubules. Water permeability in such cells is simply too high to be accounted for by lipid-mediated diffusion, thus leading biophysicists to predict that water-selective channels must exist. The search for water channel began not surprisingly in tissues that had been already identified from physiological studies as having high water permeabilities. But the molecular basis of water channels remained elusive for a long time, since many attempts to determine its structure by biochemical approaches and expression cloning were unsuccessful. The reasons of this failure were linked to the inability of the water channel to be labeled by its substrate, the lack of highly specific inhibitors and the basal diffusional permeability of cell membranes.

Keywords

Permeability Crystallization Glycerol Mercury Cysteine 

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References

  1. Agre P., Preston G.M., Smith B.L., Jung J.S., Raina S., Moon C., Guggino W.B. and Nielsen S., Am. J. Physiol. 265 (1993) F463-F476.Google Scholar
  2. Beuron F., Le Cahérec F., Guillam M.T., Cavalier A., Garret A., Tassan J.P., Delamarche C., Schultz P., Mallouh V., Rolland J.P., Hubert J.F., Gouranton J. and Thomas D., J. Biol. Chem. 270 (1995) 17414–17422.CrossRefGoogle Scholar
  3. Cheng A., van Hoek A.N., Yeager M., Verkman A.S. and Mitra A.K., Nature 387 (1997) 627–630.ADSCrossRefGoogle Scholar
  4. Gorin M.B., Yancey S.B., Cline J., Revel J.P. and Horwitz J., Cell. 39 (1984) 49–59.CrossRefGoogle Scholar
  5. Gouranton J., J. Microsc. Paris 7 (1968) 559–574.Google Scholar
  6. Hubert J.F., Thomas D., Cavalier A. and Gouranton J., Biol. Cell. 66 (1989) 155–163.Google Scholar
  7. Jap B. K. and Li H., J. Mol. Biol. 251 (1995) 413–420.CrossRefGoogle Scholar
  8. Jung J.S., Preston G.M., Smith B.L., Guggino W.B. and Agre P., J. Biol. Chem. 269 (1994) 14648–14654.Google Scholar
  9. Li H., Lee S. and Jap B.K., Nature Struct. Biol. 4 (1997) 263–265.CrossRefGoogle Scholar
  10. Le Cahérec F., Bron P., Verbavatz J.M., Garret A., Morel G., Cavalier A., Bonnec G., Thomas D., Gouranton J. and Hubert J.F., J. Cell. Sci. 109 (1995a) 1285–1295.Google Scholar
  11. Le Cahérec F., Deschamps S., Delamarche C., Pellerin I., Bonnec G., Guillam M.T., Thomas D., Gouranton J. and Hubert J.F., Eur. J. Biochem. 241 (1995b) 707–715.CrossRefGoogle Scholar
  12. Mitra A.K., van Hoek A.N., Wiener M.C., Verkman A.S. and Yeager M., Nature Struct. Biol. 2 (1995) 726–729.CrossRefGoogle Scholar
  13. Preston G.M., Caroll T.P., Guggino W.B. and Agre P., Science 256 (1992) 385–387.ADSCrossRefGoogle Scholar
  14. Reizer J., Reizer A. and Saier M.H., Crit. Rev. Biochem. Mol. Biol. 28 (1993) 235–257.CrossRefGoogle Scholar
  15. Thomas D., Schultz P., Steven A.C. and Wall J., Biol. Cell. 80 (1994) 181–192.Google Scholar
  16. Walz T., Smith B.L., Zeidel, Engel A. and Agre P., J. Biol. Chem. 269 (1994) 1583–1586.Google Scholar
  17. Walz T., Typke D., Smith B.L., Agre P. and Engel A., Nature Struct. Biol. 2 (1995) 730–732.CrossRefGoogle Scholar
  18. Walz T., Hirai T., Murata K., Heymann J.B., Mitsuoka K., Fujiyoshi Y., Smith B.L., Agre P. and Engel A., Nature 387 (1997) 624–627.ADSCrossRefGoogle Scholar

Copyright information

© Springer-Verlag France 1998

Authors and Affiliations

  • P. Bron
    • 1
  • V. Lagrée
    • 1
  • A. Froger
    • 1
  • I. Pellerin
    • 1
  • S. Deschamps
    • 1
  • J.-F. Hubert
    • 1
  • C. Delamarche
    • 1
  • A. Cavalier
    • 1
  • J.-P. Rolland
    • 1
  • J. Gouranton
    • 1
  • D. Thomas
    • 1
  1. 1.Canaux et Récepteurs Membranaires, UPRES-A 6026 du CNRS, Biologie Cellulaire et ReproductionUniversité de RenneslRennes CedexFrance

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