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Virus-Resistant Transgenic Plants: Potential Ecological Impact pp 52–65Cite as

Behavior of Cucumovirus Pseudorecombinant and Recombinant Strains in Solanaceous Hosts

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Abstract

Pseudorecombinant and chimeric recombinant strains have been created between two strains of cucumber mosaic virus (I17F-CMV and R-CMV) or between R-CMV and tomato aspermy virus (P-TAV), and their biological properties have been assessed on certain solanaceous hosts. Results involving the two CMV strains suggest that the different hosts fall into two main groups. The first corresponds to hosts for which the nature or the gravity of the symptoms is mainly determined by RNAs 1 and 2. The second group is composed of hosts for which symptomatology is mainly governed by RNA 3. In these cases, it is the 3′ part of the molecule, bearing the coat protein (CP) gene, which is primarily involved. One chimeric recombinant CMV strain has distinctive properties, since it cumulates the severe symptoms induced by both parental strains in Nicotiana glutinosa. Pseudorecombinants and recombinants created between R-CMV and P-TAV confirmed the importance of the 3′ part of RNA 3 in determining symptomatology in this host. Unexpected results were observed with one chimeric recombinant, which proved unable to infect tobacco, although it can replicate in tobacco cells. The pseudorecombinant strain composed of P-TAV RNAs 1 and 2 and RNA 3 from R-CMV induced considerably aggravated symptoms on Nicotiana benthamiana. This property is not related to a difference in replication efficiency.

Keywords

  • Coat Protein
  • Recombinant Strain
  • Cucumber Mosaic Virus
  • Coat Protein Gene
  • Severe Stunting

These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Author information

Authors and Affiliations

  1. Station de Pathologie Végétale, INRA, BP 94, 84143, Montfavet Cedex, France

    Mireille Jacquemond & Isabelle Carrère

  2. Institute for Plant Sciences, Agricultural Biotechnology Center, POB 411, 2101, Gödöllö, Hungary

    Katalin Salánki & Ervin Balázs

  3. Laboratoire de Biologie Cellulaire, INRA, 78026, Versailles Cedex, France

    Mark Tepfer

Authors
  1. Mireille Jacquemond
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  2. Katalin Salánki
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  3. Isabelle Carrère
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  4. Ervin Balázs
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  5. Mark Tepfer
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Editor information

Editors and Affiliations

  1. Laboratoire de Biologie Cellulaire, INRA-Versailles, 78026, Versailles Cedex, France

    Dr. Mark Tepfer

  2. Institute for Plant Sciences, Agricultural Biotechnology Center, POB 411, 2101, Gödöllö, Hungary

    Dr. Ervin Balázs

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© 1997 Springer-Verlag Berlin Heidelberg

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Jacquemond, M., Salánki, K., Carrère, I., Balázs, E., Tepfer, M. (1997). Behavior of Cucumovirus Pseudorecombinant and Recombinant Strains in Solanaceous Hosts. In: Tepfer, M., Balázs, E. (eds) Virus-Resistant Transgenic Plants: Potential Ecological Impact. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-03506-1_7

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  • DOI: https://doi.org/10.1007/978-3-662-03506-1_7

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