Abstract
The plasmalemma or plasma membrane surrounds the yeast cell and is visible in an electron micrograph as a dark double-layered cellular boundary (Fig. 7.1). It comprises a hydrophobic middle layer within a hydrophilic mantle (the electron-dense layers). Biological membranes separate compartments and allow a controlled exchange of solutes between them. The plasma membrane encloses the cell and controls the selective uptake of nutrients and extrusion of metabolic products. The nucleus and mitochondria are enclosed by double membranes; the vacuoles, Golgi complex, and other vesicles by single ones. An endoplasmic reticulum is present. The subcellular membranes each perform specific tasks during metabolism.
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References
Anraku Y, Umemoto N, Hirata R, Wada Y (1989) Structure and function of the yeast vacuolar membrane proton ATPase. J Bioenerg Biomembr 21: 589–603
Becker DM, Guarente L (1991) High efficiency transformation of yeast by electroporation. Methods Enzymol 194: 182–187
Dayson H, Danielli JF (1943) The permeability of natural membranes. Cambridge Univ. Press, Cambridge and New York
Driessen AJM, Konings WN (1993) Insertion of lipids and proteins into bacterial membranes by fusion with liposomes. Methods Enzymol 221: 394–408
Evans WH, Graham JM (1989) Membrane structure and function. IRL Press at Oxford University Press, Oxford
Gläser H-U, Höfer M (1987) Ion-dependent generation of the electrochemical proton gradient 0µ ir in reconstituted plasma membrane vesicles from the yeast Metschnikowia reukaufti. Biochim Biophys Acta 905: 287–294
Goffeau A (1993) Transport ATPases and yeast metabolism. In: Scheffers WA, van Dijken JP (eds) Metabolic compartmentation in yeast. Pasmans, The Hague, pp 42–44
Goffeau A, Green NM (1990) The H’-ATPase from fungal plasma membrane. In: Pasternak CA (ed) Monovalent cations in biological systems. CRC Press, Boca Raton, pp 155–169
Goswitz VC, Brooker RJ (1995) Structural features of the uniporter/symporter/antiporter superfamily. Protein Sci 4: 534–537
Gustin MC, Martinac B, Saimi Y, Culbertson MR, Kung C (1986) Ion channels in yeast. Science 233: 1195–1197
Harold FM (1986) The vital force: a study of bioenergetics. W.H. Freeman and Company, New York
Hille B (1992) Ionic channels of excitable membranes. Sinauer Associates Inc., Sunderland, MA
Higgins CF (1992) ABC transporters: from microorganisms to man. Annu Rev Cell Biol 8: 67–113
Höfer M (1981) Transport across biological membranes. Pitman Publish Ltd, London
Höfer M (1989) Accumulation of electroneutral and charged carbohydrates by proton cotransport in Rhodotorula. Methods Enzymol 174: 623–653
Höfer M, Gläser H-U (1989) K’ ions stimulate both ATPase activity and ApH generation in reconstituted yeast plasma membrane vesicles. In: Kotyk A, Skoda J, Paces V, Kostka V (eds) Highlights of modern biochemistry. VPS International, Utrecht, vol I, pp 753–760
Höfer M, Mair T, Wernsdörfer E (1991) Reconstituted plasma membrane vesicles: a tool to study transport in yeast. In: Prasad R (ed) Yeast molecular biology and biotechnology. Omega Sci Publ, New Delhi, pp 239–253
Höfer M, Calahorra M, Klein B, Pana A (1995) Assessment of AfH in Schizosaccharomyces pombe; intracellular inclusion of impermeable agents by electroporesis. Folia Microbiol 40: in press
Hofstee BHJ (1959) Non-inverted versus inverted plots in enzyme kinetics. Nature 184: 1296–1298
Ko CH, Liang H, Gaber RF (1993) Roles of multiple glucose transporters in Saccharomyces cerevisiae. Mol Cell Biol 13: 638–648
Kyte J, Doolittle RF (1982) A simple method for displaying the hydropathic character of a protein. J Mol Biol 157: 105–132
van Leeuwen CCM, Postma E, van den Broek PJA, van Steveninck J (1991) Proton-motive force-driven n-galactose transport in plasma membrane vesicles from the yeast Kluyveromyces marxianus. J Biol Chem 226: 12146–12151
Lichtenberg H-C, Giebeler H, Höfer M (1988) Measurements of electrical potential differences across yeast plasma membranes with microelectrodes are consistent with values from steady-state distribution of tetraphenylphosphonium in Pichia humboldtii. J Membr Biol 103: 255–261
Mair T, Höfer M (1988) ATP-induced generation of pH gradient and/or membrane potential in reconstituted plasma membrane vesicles from Schizosaccharomyces porn be. Biochem Int 17: 593–604
Marger MD, Saier MH Jr (1993) A major superfamily of transmembrane facilitators that catalyze uniport, symport and antiport. Trends Biochem Sci 18: 13–20
Milbradt B, Höfer M (1995) Glucose-transport-deficient mutants of Schizosaccharomyces pombe: phenotype, genetics and use for genetic complementation. Microbiol 140: 2617–2623
Michaelis L, Menten ML (1913) Kinetics of invertase action. Biochem Z 49: 333–369
Mitchell P (1966) Chemiosmotic coupling in oxidative and photosynthetic phosphorylation. Biol Rev 41: 445–502
Özcan S, Johnston M (1995) Three different regulatory mechanisms enable yeast hexose transporter (HXT) genes to be induced by different levels of glucose. Mol Cell Biol 15: 1564–1572
Pena A, Ramirez J, Rosas G, Calahorra M (1995) Proton pumping and the internal pH of yeast cells, measured with pyranine introduced by electroporation. J Bacteriol 177: 1017–1022
Prasad R (1985) Lipids in the structure and function of yeast membranes. Adv Lipid Res 21: 187–242
Reifenberger E, Freidel K, Ciriacy M (1995) Identification of novel HXT genes in Saccharomyces cerevisiae reveals the impact of individual hexose transporters on glycolytic flux. Mol Microbiol 16: 157–167
Robertson JD (1964) Unit membranes: a review with recent new studies of experimental alterations and a new subunit structure in synaptic membranes. In: Locke M (ed) Cellular membranes in development. Academic Press, New York, pp 1–81
Rose MD (1987) Isolation of genes by complementation in yeast. Methods Enzymol 152: 481–511
Smith C, Wood EJ (1991) Energy in biological systems. Chapman and Hall, London
Singer SJ, Nicolson GL (1972) The fluid mosaic model of the structure of cell membranes. Science 175: 720–731
Vacata V, Höfer M, Larsson HP, Lecar H (1993) Ionic channels in the plasma membrane of Schizosaccharomyces pombe: evidence from patch-clamp measurements. J Bioenerg Biomembr 24: 43–53
Van der Rest ME, Kamminga AH, Nakano A, Anraku Y, Poolman B, Konings WN (1995) The plasma membrane of Saccharomyces cerevisiae: structure, function and biosynthesis. Microbiol Rev 59: 304–322
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Höfer, M. (1997). Membranes. In: Spencer, J.F.T., Spencer, D.M. (eds) Yeasts in Natural and Artificial Habitats. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-03370-8_8
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DOI: https://doi.org/10.1007/978-3-662-03370-8_8
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