Micropropagation of Mammillaria Species (Cactaceae)

  • A. Rubluo
Part of the Biotechnology in Agriculture and Forestry book series (AGRICULTURE, volume 40)


The genus Mammillaria, the largest in the Cactaceae family, constitutes one of the most popular groups of cacti. This popularity lies basically in the huge variability of the genus, in its forms, color of flowers, its small size, and relative ease of culture and maintenance, all of which make it a horticultural favorite. Unfortunately, these factors mean that many species of the group are threatened with extinction. The following sections present a brief description of this genus.


Somatic Embryogenesis Embryogenic Callus Multiple Shoot Crassulacean Acid Metabolism Shoot Proliferation 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Anonymous (1994) CITES NEWS-PLANTS. A newsletter for the European region of the CITES plants committee, Issue 1Google Scholar
  2. Ault JR, Blackmon WJ (1985) In vitro propagation of selected native cacti species. Hortic Sci 20: 541 (Abstr)Google Scholar
  3. Bravo H (1978) Las cactáceas de México, vol II. Capítulo XV, subtribu III. Cactinae. Universidad Nacional Autónoma de México (in Spanish)Google Scholar
  4. Cheema GS, Mehra PN (1981) Anther culture of a cactus. Nat Cactus Succulent J 36(1): 8–11Google Scholar
  5. Clayton PW, Hubstenberger JF, Phillips GC, Butler-Nance SA (1990) Micropropagation of members of the Cactaceae subtribe Cactinae. J Am Soc Hortic Sci 115(2): 337–343Google Scholar
  6. Corneanu MN, Corneanu GC, Copacescu SN (1990) Plant regeneration with somaclonal variability from Mammillaria sp. callus. VII Int Congr Plant Tissue Cell Cult Abstr, Amsterdam, p 95Google Scholar
  7. Damiano C, Curir P, Cosmi T, Ruffoni B (1986) Tissue culture of Mammillaria spp. Hortic Sci 2(3): 804 (Abstr)Google Scholar
  8. Evans SR, Hofmann A (1979) Plants of the gods. McGraw-Hill, MaidenheadGoogle Scholar
  9. Fay MF, Gratton J (1992) Tissue culture of cacti and others succulents: a literature review and a report on micropropagation at Kew. Bradleya 10: 33–48Google Scholar
  10. Fearn B, Pearcy L (1979) 50 choice mammillarias. Abbey Brook Cactus Nursery, Matlock, DerbyshireGoogle Scholar
  11. George EF, Sherrington PD (1984) Plant propagation by tissue culture. Exegetics Limited, EversleyGoogle Scholar
  12. Havel L, Kolar Z (1983) Microexplant isolation from Cactaceae. Plant Cell Tissue Organ Cult 2: 349–353CrossRefGoogle Scholar
  13. Hubstenberger JF, Clayton PW, Phillips GC (1992) Micropropagation of cacti (Cactaceae). In: Bajaj YPS (ed) Biotechnology in agriculture and forestry, vol 20. High-tech and micropropagation IV. Springer, Berlin Heidelberg New York, pp 49–68Google Scholar
  14. Hunt D (1992) CITES Cactaceae checklist. Royal Botanical Gardens, KewGoogle Scholar
  15. IUCN (1985) Rare and threatened plant list. Threatened Plant Committee Botanic Garden Conservation, Coordinating Body, KewGoogle Scholar
  16. Johnson JL, Emino ER (1979) In vitro propagation of the cactus Mammillaria elongata. Hortic Sci 14: 605–606Google Scholar
  17. Kolar Z, Bartek J, Viskot B (1976) Vegetative propagation of the cactus Mammillaria woodsii through tissue cultures. Experientia 32: 668–669CrossRefGoogle Scholar
  18. MartInez-Vázquez O, Rubluo A (1989) In vitro mass propagation of the near-extinct Mammillaria san-angelensis Sánchez-Mejorada. J Hortic Sci 64(1): 99–105Google Scholar
  19. Mauseth JD (1979) A new method for the propagation of cacti: sterile culture of axillary buds. Cactus Succulent J 51: 186–187Google Scholar
  20. Minocha SC, Mehra PN (1974) Nutritional and morphogenetic investigations of callus cultures of Neomammillaria prolifera Miller (Cactaceae). Am J Bot 61: 168–173CrossRefGoogle Scholar
  21. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15: 473–497CrossRefGoogle Scholar
  22. Oldfield S (1985) Whiter international trade in plants? New Sci 106: 10–11Google Scholar
  23. Reyes JS (1994) Metodos para la propagación de Cactáceas Mexicanas. Amaranto 7(2): 1–12(in Spanish)Google Scholar
  24. Rodríguez-Garay B, Rubluo A(1992) In vitro morphogenetic responses of the endangered cactus Aztekium ritteri (Boedeker). Cactus Succulent J 64: 116–119Google Scholar
  25. Rubluo A, Arriaga E, Arias S, Pérez-Amador C, Amor D, Santos E, Rojas E, Elizalde P (1990) Tissue culture applications in the endangered Mammillaria hritsilopochlti (Cactaceae). VII Int Congr Plant Tissue Cell Cult Abstr, Amsterdam, p 130Google Scholar
  26. Rubluo A, Rodríguez-Garay B, Roa A, Duval K (1992) Micropropagation and in vitro production of novelties of commercially attractive endangered cacti. 1992 In vitro culture and horticultural breeding, Abstr, Baltimore, p 65Google Scholar
  27. Rubluo A, Chávez V, Martínez AP, Martínez-Vázquez O (1993) Strategies for the recovery of endangered orchids and cacti through in vitro culture. Biol Conserv 63: 163–169CrossRefGoogle Scholar
  28. Rubluo A, Novak F, Brunner H, Van Duren M, Marquez J, Duval K, Marin T (1994) Conservation and generation of plant diversity through tissue culture in endangered species. VIII Int Congr Plant Tissue Cell Cult Abstr, Florence, p 116Google Scholar
  29. SEDESOL (1994) Norma oficial Mexicana de especies en extinción. Diario Oficial de la Federación Tomo CDLXXXVIII No 10, pp 10–13 (in Spanish)Google Scholar
  30. Starling RJ, Dodds JH (1983) Tissue culture propagation of cacti and other succulents. Bradleya 1: 84–90Google Scholar
  31. Stuppy W, Nagl W (1992) Regeneration and propagation of Ariocarpus retusus Scheidw. (Cactaceae) via somatic embryogenesis. Bradleya 10: 85–88Google Scholar
  32. Tisserat B, Esan EB, Murashige T(1979) Somatic embryogenesis in angiosperms. In: Janick J (ed) Horticultural reviews, vol I. AVI, Westport, Connecticut, pp 1–78Google Scholar
  33. Vyskot B, Jara Z (1984) Clonal propagation of cacti through axillary buds in vitro. J Hortic Sci 59: 449–452Google Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1997

Authors and Affiliations

  • A. Rubluo
    • 1
  1. 1.The Botanical Garden, Institute of BiologyUNAMMexicoD. F. Mexico

Personalised recommendations