Cryopreservation Studies on Digitalis lanata (Foxglove)

  • U. Seitz
Part of the Biotechnology in Agriculture and Forestry book series (AGRICULTURE, volume 32)

Abstract

Plants of the genus Digitalis (foxglove, Scrophulariaceae) are biennial or perennial herbs, and belong to the Eurasian flora. D. lanata is found especially in southeast Europe, for example in Hungary and in the Balkan countries. It has become naturalized in North America. D. purpurea is not only a medicinal plant; as an ornamental it is widespread in western and central Europe. Like D. lanata,it has become naturalized in North America. Other important species are D. grandiflora and D. lutea.

Keywords

Sugar Crystallization Sucrose Glycerol Europe 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Alfermann AW, Bergmann W, Figur C, Helmbold U, Schwantag D, Schuller I, Reinhard E (1983) Biotransformation of ß-methyldigitoxin to ß-methyldigoxin by cell cultures of Digitalis lanata. In: Mantell SH, Smith H (eds) Plant biotechnology. Cambridge Univ Press, Cambridge, pp 6774Google Scholar
  2. Bajaj YPS (1976) Regeneration of plants from cell suspensions frozen at — 20, — 70, and — 196 °C. Physiol plant 37: 263–268CrossRefGoogle Scholar
  3. Bajaj YPS (1988) Cryopreservation and the retention of biosynthetic potential in cell cultures of medicinal and alkaloid-producing plants. In: Bajaj YPS (ed) Biotechnology in agriculture and forestry, vol 4. Medicinal and aromatic plants I. Springer, Berlin Heidelberg New York, pp 169–187Google Scholar
  4. Bhandal IS, Hauptmann RM, Widholm JM (1985) Trehalose as cryoprotectant for the freeze preservation of carrot and tobacco cells. Plant Physiol 78: 430–432PubMedCrossRefGoogle Scholar
  5. Diettrich B, Popov AS, Pfeiffer B, Neumann D, Butenko R, Luckner M (1982) Cryopreservation of Digitalis lanata cell cultures. Planta Med 46: 82–87PubMedCrossRefGoogle Scholar
  6. Diettrich B, Haack U, Popov AS, Butenko RG, Luckner M (1985) Long-term storage in liquid nitrogen of an embryogenic cell strain of Digitalis lanata. Biochem Physiol Pflanz 180: 33–43Google Scholar
  7. Diettrich B, Haack U, Luckner M (1986) Cryopreservation of Digitalis lanata cells grown in vitro. Precultivation and recultivation. J Plant Physiol 126: 63–73Google Scholar
  8. Diettrich B, Haack U, Thom F, Matthes G, Luckner M (1987a) Influence of intracellular ice formation on the survival of Digitalis lanata cells grown in vitro. Cryo Lett 8: 98–107Google Scholar
  9. Diettrich B, Wolf T, Bormann A, Popov AS, Butenko RG, Luckner M (1987b) Cryopreservation of Digitalis lanata shoot tips. Planta Med 53: 359–363PubMedCrossRefGoogle Scholar
  10. Diettrich B, Neumann D, Luckner M (1991) Cryopreservation of Digitalis lanata cells. Membrane area reduction of the protoplast during cryoprotector treatment and cooling. J Plant Physiol 139: 212–217Google Scholar
  11. Dougall DK, Whitten GH (1980) The ability of wild carrot cell cultures to retain their capacity for anthocyanin synthesis after storage at —140 °C. Planta Med (Suppl): 129–135Google Scholar
  12. Erdei I, Kiss Z, Maliga P (1981) Rapid clonal multiplication of Digitalis lanata in tissue culture. Plant Cell Rep 1: 34–35CrossRefGoogle Scholar
  13. Gautheret RJ (1959) La culture des tissus végétaux. Techniques et réalisations. Masson, ParisGoogle Scholar
  14. Göldner EM, Seitz U, Reinhard E (1991) Cryopreservation of Digitalis lanata Ehrh. cell cultures: preculture and freeze tolerance. Plant Cell Tissue Organ Cult 24: 19–24CrossRefGoogle Scholar
  15. Kuberski C, Scheibner H, Steup C, Diettrich B, Luckner M (1984) Embryogenesis and cardenolide formation in tissue cultures of Digitalis lanata. Phytochemistry 23: 1407–1412CrossRefGoogle Scholar
  16. Luckner M, Diettrich B (1992) Micropropagation of woolly foxglove (Digitalis lanata) by shoot tip culture. In: Bajaj YPS (ed) Biotechnology in agriculture and forestry, vol 19. High-tech and micropropagation III. Springer, Berlin Heidelberg New York, pp 400–424Google Scholar
  17. Reuff I, Seitz U, Ulbrich B, Reinhard E (1988) Cryopreservation of Coleus blumei suspension and callus cultures. J Plant Physiol 133: 414–418CrossRefGoogle Scholar
  18. Rücker W (1988) Digitalis spp.: in vitro culture, regeneration, and the production of cardenolides and other secondary products. In: Bajaj YPS (ed) Biotechnology in agriculture and forestry, vol 4. Medicinal and aromatic plants I. Springer, Berlin Heidelberg New York, pp 388–418Google Scholar
  19. Scheibner H, Björk L, Schulz U, Diettrich B, Luckner M (1987) Influence of light on cardenolide accumulation in somatic embryos of Digitalis lanata. J Plant Physiol 130: 211–219CrossRefGoogle Scholar
  20. Seitz U, Alfermann AW, Reinhard E (1983) Stability of biotransformation capacity in Digitalis lanata cell cultures after cryogenic storage. Plant Cell Rep 2: 273–276CrossRefGoogle Scholar
  21. Seitz U, Reuff I, Reinhard E (1985) Cryopreservation of plant cell cultures. In: Neumann KH, Barz W, Reinhard E (eds) Primary and secondary metabolism of plant cell cultures. Springer, Berlin Heidelberg New York, pp 323–333CrossRefGoogle Scholar
  22. Sepasgosarian J (1987) 16ß-Hydroxylierung durch Zellkulturen von Digitalis purpurea L. Thesis, Univ Tübingen, pp 6–8Google Scholar
  23. Steponkus PL, Dowgert MF, Gordon-Kamm WJ (1983) Destabilization of the plasma membrane of isolated plant protoplasts during a freeze-thaw cycle: the influence of cold acclimation. Cryobiology 20: 448–465PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1995

Authors and Affiliations

  • U. Seitz
    • 1
  1. 1.Botanical InstituteUniversity of TübingenTübingenGermany

Personalised recommendations