Conservation of Germplasm of Strawberry (Fragaria Species)

  • B. M. Reed
  • K. E. Hummer
Part of the Biotechnology in Agriculture and Forestry book series (AGRICULTURE, volume 32)


The genus Fragaria L. is a member of the Rosaceae and subfamily Rosoidea. The base chromosome number is x = 7, and the genus includes 17 generally accepted diploid, tetraploid, hexaploid, and octoploid species (Table 1). This genus is native to temperate locations in the northern hemisphere and South America. There are no endangered species at this time. Fragaria can be found at elevations from sea level through 4000 m. Fragaria vesca L., the wood strawberry, is the most extensively distributed species of this genus. Hybridization between Fragaria and a related genus Potentilla is possible, although most hybrids are sterile.


Cold Storage Cold Hardening Strawberry Cultivar Wild Strawberry Strawberry Production 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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  1. Angelo E (1939) The development of cold resistance in strawberry varieties. Minn Agric Exp Stn Tech Bul 135: 7–19Google Scholar
  2. Anonymous (1990) Agricultural Statistics. US Dep Agric, Gov Printing Office, Washington, DCGoogle Scholar
  3. Bajaj YPS (1985) Cryopreservation of germplasm of potato (Solanum tuberosum L.) and cassava (Manihot esculenta Crantz): Viability of excised meristems cryopreserved up to 4 years. Indian J Exp Bio123: 285–287Google Scholar
  4. Bajaj YPS (1991) Storage and cryopreservation of in vitro cultures. In: Bajaj YPS (ed) Biotechnology in agriculture and forestry, vol 17. High-tech and micropropagation I. Springer, Berlin Heidelberg New York, pp 361–381Google Scholar
  5. Bettencourt EJ, Konopka J (eds) (1989) Directory of germplasm collections 6.11 Temperate fruits and tree nuts. Int Board Plant Genet Resour, RomeGoogle Scholar
  6. Boxus P (1976) The production of virus-free strawberry by in vitro culture. Acta Hortic 66: 35–38Google Scholar
  7. Bringhurst R, Voth V (1957) Effect of stratification on strawberry seed germination. Proc Am Soc Hortic Sci 92: 369–375Google Scholar
  8. Converse R (1987) Detection and elimination of virus and viruslike diseases in strawberry. In: Converse R (ed) Virus diseases of small fruits. USDA Agric Handbook No 631: 1–100Google Scholar
  9. Craddock WJH (1987) Cryopreservation of pollen. MS Thesis, Oregon State University, 77 ppGoogle Scholar
  10. Damiano C (1979) Cold storage of in vitro strawberry cultures and the resumption of multiplication. Ann Ist Sper Fruttic 10: 53–58Google Scholar
  11. Dereuddre J, Scottez C, Arnaud Y, Duron M (1990) Resistance d’apex de vitroplants de poirier (Pyrus communis L. cv. Beurré Hardy) enrobés dans l’alginate, à une deshydration puis à une congélation dans l’azote liquide: effet d’un endurcissement préalable au froid. CR Acad Sci Paris 310: 317–323Google Scholar
  12. Finkle BJ, Ulrich JM (1979) Effects of cryoprotectants in combination on the survival of frozen sugarcane cells. Plant Physiol 63: 598–604PubMedCrossRefGoogle Scholar
  13. Gunning J, Lagerstedt HB (1985) Long-term storage techniques for in vitro plant germplasm. Proc Int Plant Prop Soc 35: 199–205Google Scholar
  14. Hancock JF, Maas JL, Shanks CH, Breen PJ, Luby JJ (1990) Strawberries (Fragaria) In: Moore JN, Ballington JR Jr (eds) Genetic resources of temperate fruit and nut crops II. Acta Hortic 290: 489–546Google Scholar
  15. Hancock JF, Luby JJ (1993) Genetic resources at our doorstep: the wild strawberries. Science 43 (3): 141–147Google Scholar
  16. Hummer K (1991) Fragaria at the National Clonal Germplasm Repository at Corvallis, Oregon. In: Dale A, Luby JJ (eds) The strawberry into the 21st century. Timber Press, Portland, OR, pp 106–107Google Scholar
  17. Jungnickel F (1988) Strawberries (Fragaria spp. and hybrids). In: Bajaj YPS (ed) Biotechnology in agriculture and forestry, vol 6. Crops II. Springer, Berlin Heidelberg New York, pp 38–103Google Scholar
  18. Kartha KK (1985) Cryopreservation of plant cells and organs. Newsl Int Assoc Plant Tissue Cult 45: 2–15Google Scholar
  19. Kartha KK, Leung NL, Pahl K (1980) Cryopreservation of strawberry meristems and mass propagation of plantlets. J Am Soc Hortic Sci 105 (4): 481–484Google Scholar
  20. Luby J, Hancock J, Cameron JS (1991) Expansion of the strawberry germplasm base in North America. In: Dale A, Luby JJ (eds) The strawberry into the 21st century. Timber Press, Portland, Oregon, pp 66–75Google Scholar
  21. Maas J (1984) Compendium of strawberry diseases. Am Phytopathol Soc, St. Paul, MN Mass J, Galletta G (1993) eds 2nd Int Strawberry Symp. Acta Hortic 348: 1–520Google Scholar
  22. Marino G, Rosati P, Sagrati F (1985) Storage of in vitro cultures of Prunus rootstocks. Plant Cell Tissue Organ Cult 5: 73–78CrossRefGoogle Scholar
  23. Moore J (1991) The strawberry into the 21st century: a symposium overview. In: Dale A, Luby JJ (eds) The strawberry into the 21st century. Timber Press, Portland, OregonGoogle Scholar
  24. Moore P, Robbins J, Sjulin T (1991) Field performance of olympus strawberry subclones. Hortsci 26 (2): 192–194Google Scholar
  25. Mullin RH, Schlegel DE (1976) Cold storage maintenance of strawberry meristem plantlets. HortScience 11 (2): 100–101Google Scholar
  26. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15: 473–497CrossRefGoogle Scholar
  27. Nord SM, Hanniford GG (1989) In vitro storage of Pelargonium x domesticum. Am Soc Hortic Sci Annu Meet Program and Abstr 158 (Abstr): 78Google Scholar
  28. Reed BM (1988) Cold acclimation as a method to improve survival of cryopreserved Rubus meristems. Cryo Lett 9: 166–171Google Scholar
  29. Reed BM (1990) Survival of in vitro-grown apical meristems of Pyrus following cryopreservation. HortScience 25: 111–113Google Scholar
  30. Reed BM (1991) Application of gas-permeable bags for in vitro cold storage. Plant Cell Rep 10: 431–434Google Scholar
  31. Reed BM (1992) Cold storage of strawberries in vitro: a comparison of three storage systems. Fruit Var J 46 (2): 98–102Google Scholar
  32. Sakai A, Yamakawa M, Sakata D, Harada T, Yakuwa T (1978) Development of a whole plant from an excised strawberry runner apex frozen to —196 °C. Low Temp Sci Ser B 36: 31–38Google Scholar
  33. Scott D, Draper A (1967) Light in relation to seed germination of blueberries, strawberries and Rubus. HortScience 2 (3): 107–108Google Scholar
  34. Scott D, Draper A (1970) A further note on longevity of strawberry seed in cold storage. HortScience 5 (5): 439Google Scholar
  35. Scott D, Hancock JF (1989) Strawberry cultivars and worldwide patterns of strawberry production. Fruit Var J 42: 102–108Google Scholar
  36. Sjulin T, Dale A (1987) Genetic diversity of North American strawberry cultivars. J Am Soc Hortic Sci 112: 375–386Google Scholar
  37. Sullivan JA (1991) Improvement of strawberry using tissue culture techniques. In: Dale A, Luby JJ (ed) The strawberry into the 21st century, Timber Press, Portland, OR pp 84–90Google Scholar
  38. Swartz H, Galletta G, Zimmerman R (1981) Field performance and phenotypic stability of tissueGoogle Scholar
  39. culture-propagated strawberries. J Am Soc Hortic Sci 106(5): 667–673Google Scholar
  40. Towill L (1988) Survival of shoot tips from mint species after short-term exposure to cryogenic conditions. HortScience 23: 839–841Google Scholar
  41. Wilhelm S, Sagen J (1974) A history of the strawberry. Univ California, BerkeleyGoogle Scholar
  42. Withers LA (1986) In vitro approaches to the conservation of plant genetic resources. In: Withers LA, Alderson FG (eds) Plant tissue culture and its agricultural applications. Butterworths, London, pp 261–276Google Scholar
  43. Yamada T, Sakai A, Matsumura T, Higuchi S (1991) Cryopreservation of apical meristems of white clover (Trifolium repens L.) by vitrification. Plant Sci 78: 81–87CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1995

Authors and Affiliations

  • B. M. Reed
  • K. E. Hummer
    • 1
  1. 1.National Clonal Germplasm RepositoryUSDA/ARSCorvallisUSA

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