Skip to main content

Cryopreservation of Alfalfa (Medicago sativa L.) and Clovers (Trifolium Species)

  • Chapter

Part of the book series: Biotechnology in Agriculture and Forestry ((AGRICULTURE,volume 32))

Abstract

Alfalfa (Medicago sativa L.) and clovers (white clover — Trifolium repens L. and red clover — Trifolium pratense L.) are the most important agronomic forage legumes and soil improvement crops in the temperate areas of the world. They are perennial, and are cultivated for their vegetable matter, fresh or preserved, which is used as feed for animals. The origin of alfalfa is in Iran (see Bolton et al. 1972), and of red and white clovers in Europe. Economically, white clover is more important than red clover. White clover is well adapted to cooler climates and to poor soils. Medicago and Trifolium species are the most useful culture as forage grasses, because their pasture value is much more important than that of red clover (after McCoy and Walker 1984; Phillips and Collins 1984).

This is a preview of subscription content, log in via an institution.

Buying options

Chapter
USD   29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD   259.00
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD   329.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD   329.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Learn about institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  • Bajaj YPS (1983) Production of normal seeds from plants regenerated from meristems of Arachis hypogaea and Cicer arietinum cryopreserved for 20 months. Euphytica 32: 425–430

    Article  Google Scholar 

  • Bajaj YPS (1986) In vitro preservation of genetic resources. Techniques and problems. Int Symp Nuclear techniques and in vitro culture for plant improvement, 1985 IAEA, Vienna, pp 43–57

    Google Scholar 

  • Bajaj YPS (1990) Cryopreservation of germplasm of legumes and oilseed crops. In: Bajaj YPS (ed) Biotechnology in agriculture and forestry, vol 10. Legumes and oilseed crops. I. Springer, Berlin Heidelberg New York, pp. 49–62

    Google Scholar 

  • Bhojwani SS (1981) A tissue culture method for propagation and low temperature storage of Trifolium repens genotypes. Physiol Plant 52: 187–190

    Article  CAS  Google Scholar 

  • Bolton JL, Goplen BP, Baenziger H (1972) World distribution and historical developments. In: Hanson CH (ed) Alfalfa science and technology. Am Soc Agron, Madison, pp 1–34

    Google Scholar 

  • Cachitâ CD, Crâciun C (1990) Ultrastructural studies of some ornamentals In• Ammirato PV, Evans DA, Sharp WR, Bajaj YPS (eds) Handbook of plant cell culture, vol 5, Ornamental species. McGraw-Hill, New York, pp 57–94

    Google Scholar 

  • Cachitâ CD, Zâpîrtan M, Crâciun C, Râkosy TL, Vicol A, Cristea V, Varga P, Crâciun V (1991) The response of various types of cormophytoinocula to cryopreservation (— 196 °C). In: The IVth Nat Symp on Plant Cell and Tissue Culture. Romania, Cluj-Napoca, 1989, pp 90–91

    Google Scholar 

  • Cheyne VA, Dale PJ (1980) Shoot-tip culture in forage legumes. Plant Sci Lett 19: 303–309

    Article  CAS  Google Scholar 

  • Finkle BJ, Ulrich JM, Rains DM, Tisserat BB, Schaeffer GW (1979) Survival of alfalfa, rice and date palm callus after liquid nitrogen freezing. Cryobiology 16: 583

    Article  Google Scholar 

  • Finkle BJ, Ulrich JM, Rains WR, Stavarek SS (1985) Growth and regeneration of alfalfa callus lines after freezing in liquid nitrogen. Plant Sci 42: 133–140

    Article  CAS  Google Scholar 

  • McCoy T, Walker K (1984) Alfalfa. In: Ammirato PV, Evans DA, Sharp WR, Yamada Y (eds) Handbook of plant cell culture, vol 3, Crop species. MacMillan, London, pp 171–192

    Google Scholar 

  • Morariu I (1957) Genul Medicago,In: Sâvulescu T. (ed), Floraâne, Ed Acad Române, vol 5,pp 118–136

    Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15: 473–497

    Article  CAS  Google Scholar 

  • Nyarady A (1957) Genul Trifolium,In: Sâvulescu T (ed), Flora RP Române, Ed Acad Române, vol 5, pp 145–220

    Google Scholar 

  • Phillips GC, Collins GB (1984) Red clover and other forage legumes. In: Sharp WR, Evans DA, Ammirato PV, Yamada Y (eds) Handbook of plant cell culture, vol 2, Crop species. MacMillan, London, pp 169–210

    Google Scholar 

  • Rick CM (1984) Plant germplasm resources In• Ammirato PV, Evans DA, Sharp WR, Yamada Y (eds) Handbook of plant cell culture, vol 3. Crop species. MacMillan, London, pp 9–37

    Google Scholar 

  • Stanwood PC (1985) Cryopreservation of seed germplasm for genetic conservation. In: Kartha KK (ed) Cryopreservation of plant cells and organs. CRC Press, Boca Raton, Florida, pp 199–226

    Google Scholar 

  • Stanwood PC, Bass LN (1978) Ultracold preservation of germplasm. In: Li PH, Sakai A (eds) Plant cold hardiness and freezing stress. Mechanisms and crop implications. Academic Press, New York, pp 361–371

    Google Scholar 

  • Sun Long-hua, Jian Ling-cheng (1990) Cryopreservation of sainfoin tissue cultures and their ultrastructural observation. Acta Bot Sin 32 (4): 262–267

    Google Scholar 

  • Tomes DT (1979) A tissue culture procedure for propagation and maintenance of Lotus corniculatus genotypes. Can J Bot 57: 137–140

    Article  Google Scholar 

  • Withers LA (1987) Long-term preservation of plant cells, tissues and organs. Oxford Sury Plant Mol Cell Biol 4: 221–272

    Google Scholar 

  • Yamada T, Sakai A, Matsumura T, Higuchi S (1991) Cryopreservation of apical meristems of white clover (Trifolium repens L.) by vitrification. Plant Sci 78: 81–87

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 1995 Springer-Verlag Berlin Heidelberg

About this chapter

Cite this chapter

Cachită, C.D., Crăciun, C. (1995). Cryopreservation of Alfalfa (Medicago sativa L.) and Clovers (Trifolium Species). In: Bajaj, Y.P.S. (eds) Cryopreservation of Plant Germplasm I. Biotechnology in Agriculture and Forestry, vol 32. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-03096-7_19

Download citation

  • DOI: https://doi.org/10.1007/978-3-662-03096-7_19

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-08184-2

  • Online ISBN: 978-3-662-03096-7

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics