Skip to main content

Cryopreservation of Germplasm of Rubber (Hevea brasiliensis)

  • Chapter
Cryopreservation of Plant Germplasm I

Part of the book series: Biotechnology in Agriculture and Forestry ((AGRICULTURE,volume 32))

Abstract

The genus Hevea (family Euphorbiaceae) consists of nine species, namely H. brasiliensis, H. benthamiana, H. camporum, H. guianensis, H. microphylla, H. nitida, H. pauciflora, H. rigidifolia, and H. spruceana (Baldwin 1947). Of these, H. brasiliensis (Wild.) Muell.-Arg. is the only commercial species and the primary source of natural rubber. Three other species, H. benthamiana, H. pauciflora, and H. spruceana are used as sources of resistance to South American Leaf Blight (Ho 1979).

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 259.00
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 329.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 329.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  • Abdelnour-Esquivel A, Mora A, Villalabos V (1992a) Cryopreservation of zygotic embryos of Musa acuminata (AA) and M. balbisiana ( BB ). Cryo Lett 13: 159–164

    Google Scholar 

  • Abdelnour-Esquivel A, Villalabos V, Engelmann F (1992b) Cryopreservation of zygotic embryos of Coffea spp. Cryo Lett 13: 297–302

    Google Scholar 

  • Ang BB (1976) Problems of rubber seed storage. In: Chin HF, Enoch IC, Raja Harun RM (eds) Seed technology in the tropics. Univ Pertanian, Malaysia, pp 117–122

    Google Scholar 

  • Assy-Bah B, Engelmann F (1992a) Cryopreservation of immature embryos of coconut (Cocos nucifera L.). Cryo Lett 13: 67–74

    Google Scholar 

  • Assy-Bah B, Engelmann F (1992b) Cryopreservation of mature embryos of coconut (Cocos nucifera L.) and subsequent regeneration of plantlets. Cryo Lett 13: 117–126

    Google Scholar 

  • Bajaj YPS (1984) Induction of growth in frozen embryos of coconut and ovules of citrus. Curr Sci 53: 1215–1216

    Google Scholar 

  • Bajaj YPS (1985) Cryopreservation of embryos. In: Kartha KK (ed) Cryopreservation of plant cells and organs. CRC Press, Boca Raton, pp 227–242

    Google Scholar 

  • Baldwin JR JT (1947) Hevea: a first interpretation. J Hered 38: 54

    Google Scholar 

  • Chen Z (1984) Rubber (Hevea). In: Sharp WR, Evans DA, Ammirato PV, Yamada Y (eds) Handbook of plant cell culture, vol 2 Crop species. Macmillan, New York, pp 546–571

    Google Scholar 

  • Chin HF, Maheran A, Ang BB, Samsidar H (1981) The effect of moisture and temperature on the ultrastructure and viability of seeds of Hevea brasiliensis. Seed Sci Technol 9: 411–422

    Google Scholar 

  • Chin HF, Krishnapillay B, Alang ZC (1988) Cryopreservation of Veitchia and Howea palm embryos. Cryo Lett 9: 372–379

    Google Scholar 

  • Fridborg G, Pederson M, Landstrom L, Eriksson T (1978) The effect of activated charcoal on tissue cultures: absorption of metabolites inhibiting morphogenesis. Physiol Plant 43: 104–106

    Article  CAS  Google Scholar 

  • Grout BWW, Shelton K, Pritchard HW (1983) Orthodox behavior of oil palm seed and cryopreservation of the excised embryo for genetic conservation. Ann Bot 52: 381–384

    Google Scholar 

  • Ho CY (1979) Conservation and utilization of Hevea germplasm. Malays. Appl Biol 8 (1): 1–14

    Google Scholar 

  • Keleny GP, Van Haaren AJH (1976) Progress report on rubber seed investigation. Papua New Guinea Agric J 19 (2): 72–87

    Google Scholar 

  • King MW, Roberts EH (1979) The storage of recalcitrant seeds—achievement and possible approaches. Dep Agric Hortic Univ Reading, UK, pp 1–45

    Google Scholar 

  • King MW, Roberts EH (1980) Maintenance of recalcitrant seeds in storage. In: Chin HF, Roberts EH (eds) Recalcitrant crop seeds. Tropical Press, Sdn Bhd, Kuala Lumpur, pp 53–89

    Google Scholar 

  • Krishnapillay B (1989) Towards the development of a protocol for cryopreservation of embryos of a recalcitrant seed (Artocarpus heterophyllus Lam.). PhD Thesis, Univ Pertanian Malaysia, Malaysia

    Google Scholar 

  • Marin ML, Mafia G, Roca WM, Withers LA (1990) Cryopreservation of cassava zygotic embryos and whole seeds in liquid nitrogen. Cryo Lett 11: 257–264

    Google Scholar 

  • Mumford PM, Grout BWW (1978) Desiccation and low temperature (-196 °C) tolerance of Citrus limon seed. Seed Sci Technol 7: 407–410

    Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15: 473–497

    Article  CAS  Google Scholar 

  • Muzik TJ (1956) Studies on the development of the embryo and seed of Hevea brasiliensis in culture. Lloydia 19: 86–91

    Google Scholar 

  • Normah MN (1987) Effects of temperature on rubber (Hevea brasiliensis Muell.-Arg.) seed storage. PhD Thesis, Univ Pertanian Malaysia, Malaysia

    Google Scholar 

  • Normah MN, Chin HF (1989a) Effects of temperature on storage of Hevea seeds treated with polyethylene glycol 6000. Malays Appl Biol 18 (2): 133–138

    Google Scholar 

  • Normah MN, Chin HF (1989b) Recalcitrant seed storage by partial desiccation technique. Acta Hortic 253: 258–259

    Google Scholar 

  • Normah MN, Vengadasalam M (1992) Effects of moisture content on cryopreservation of Coffea and Vigna seeds and embryos. Cryo Lett 13: 199–208

    Google Scholar 

  • Normah MN, Chin HF, Hor YL (1986) Desiccation and cryopreservation of embryonic axes of Hevea brasiliensis Muell. Arg Pertanika 9 (3): 299–303

    Google Scholar 

  • Normah MN, Chin HF, Hor YL (1988) Conservation of Hevea by cryopreserved embryo culture technique. In: Umaly R, Umboh I, Halos S, Normah MN (eds) The application of tissue culture techniques in economically important tropical trees. SEAMEO — BIOTROP, Bogor, BIOTROP Spec Publ 35: 127–131

    Google Scholar 

  • Ong TP, Lauw IK (1963) Results of storage tests with seeds of Hevea brasiliensis. Menara Perkebunan 32: 183–192

    Google Scholar 

  • Paranjothy K, Ghandimathi H (1975) Morphogenesis in callus cultures of Hevea brasiliensis Muell Arg. Proc Natl Plant Tissue Culture Symp, Kuala Lumpur, pp 19–25

    Google Scholar 

  • Pence VC (1991) Cryopreservation of immature embryos of Theobroma cacao. Plant Cell Rep 10: 144–147

    Article  Google Scholar 

  • Pereira JP (1980) Conservacao da viabilidade do Poder germinativo da semente de seringueira. Pesqui Agropecu Bras Brasilia 15 (2): 237–244

    Google Scholar 

  • Roberts EH (1973) Predicting the storage life of seeds. Seed Sci Technol 1: 499–514 Rubber Research Institute of Malaya (1966) Annu Rep RRIM 1966, p 27

    Google Scholar 

  • Sakhibun MH (1981) Storage and viability of Hevea seeds. M Agric Sci Thesis, Univ Pertanian Malaysia, Malaysia

    Google Scholar 

  • Sun CN (1958) The survival of excised pea seedlings after drying and freezing in liquid nitrogen. Bot Gaz 119: 234–236

    Article  Google Scholar 

  • Toraun NL, Saryatmana N (1977) Tissue culture of Hevea brasiliensis Muell. Arg Menara Perkebunan 45: 17–21

    Google Scholar 

  • Withers LA (1979) Freeze preservation of somatic embryos and clonal plantlets of carrot (Daucus carota L.). Plant Physiol 63: 460–467

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 1995 Springer-Verlag Berlin Heidelberg

About this chapter

Cite this chapter

Normah, M.N., Chin, H.F. (1995). Cryopreservation of Germplasm of Rubber (Hevea brasiliensis) . In: Bajaj, Y.P.S. (eds) Cryopreservation of Plant Germplasm I. Biotechnology in Agriculture and Forestry, vol 32. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-662-03096-7_11

Download citation

  • DOI: https://doi.org/10.1007/978-3-662-03096-7_11

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-08184-2

  • Online ISBN: 978-3-662-03096-7

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics