Cryopreservation of Somatic Embryos

  • Y. P. S. Bajaj
Part of the Biotechnology in Agriculture and Forestry book series (AGRICULTURE, volume 30)


Since somatic embryos (SEs) can be produced in large numbers in bioreactors (Denchev et al. 1992), and are being employed for mass micropropagation (Merkle et al. 1991; Bajaj 1991a), genetic transformation (Ellis 1993), and for the production of synthetic seed (Redenbaugh et al. (1991) methods need to be developed for their storage so that they can be used when desired. Experiments have been conducted on methods such as desiccation (Gray and Purohit 1991; Tessereau et al. 1991), nonfrozen low-temperature storage (Hiraoka 1988), coating with gels (Dumet et al. 1993), and cryopreservation in liquid nitrogen (LN). This last aspect has attracted considerable attention, especially for the conservation of germplasm (see Bajaj 1986, 199lb). Somatic embryos/embryogenic cultures of a number of plant species have now been successfully cryopreserved, and plants have been obtained from retrieved cultures (Table 1). The prospects of cryopreservation of various types of embryos, i.e., zygotic, nucellar, somatic, and pollen embryos have been discussed earlier (Bajaj 1985). In this chapter, studies on the freeze-storage of SEs in liquid nitrogen are discussed.


Somatic Embryo Zygotic Embryo Freeze Storage Synthetic Seed Plant Cell Tissue Organ Cult 
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Copyright information

© Springer-Verlag Berlin Heidelberg 1995

Authors and Affiliations

  • Y. P. S. Bajaj
    • 1
  1. 1.Punjab Agricultural UniversityLudhianaIndia

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